摘要
为探究宁夏枸杞潜在的营养价值,从多酚组成的角度研究宁夏枸杞的抗氧化机理,采用四级杆串联飞行时间质谱MS^E方法(Q-TOF/MS^E)分析宁夏枸杞果实中的多酚成分,测定其提取物(LBE)中总多酚、总黄酮含量,并从细胞水平评价其抗氧化活性。结果表明,宁夏枸杞果实中共鉴定出28种多酚类化合物,其中8种酚酰胺衍生物为首次发现及鉴定。体外试验结果显示,LEB中总多酚、总黄酮含量分别为3 320 mg GAE·100g-1DW和570 mg RE·100g-1DW。进一步研究发现,LBE能有效降低氧化应激状态下人克隆结肠癌细胞(Caco-2)中活性氧自由基(ROS)水平和还原型谷胱甘肽(GSH)水平,且效果呈剂量依赖性,这从细胞水平证明LBE可以显著增强Caco-2细胞的抗氧化能力。综上,宁夏枸杞中含有大量多酚类化合物,并具有较强的抗氧化活性,可以广泛应用于营养食品、制药、化妆品以及各类天然产品中。
The polyphenols of in Lycium barbarum L. extraction （LBE） was characterized by high - resolution mass spectrometry using MSE （ Q - TOF/MSE ） data collection technology in order to study the potential nutritional value of LBE. The total polyphenols content （TPC）, total flavonoids content （TFC） and the intracellular antioxidant activity was determinated. A total of 28 polyphenols were tentatively identified, of which 8 represent the first reports of these polyphenols in LBE. we confirmed that the total polyphenols and flavonoids contents of LRF fruit extracts （LRFEs） were 3 320 mg GAE·100g-1DWand 579 mg RE · 100 g-1 DW, respectively. Furthermore, LBE was found to significantly inhibited the ROS and GSH level in Caco -2 cell treated with H202 in a dose -dependent manner. These results suggest that LBE could be applied in functional food, pharmaceutical cosmetic and other natural product industry because of the multiple polyphenols, flavonoids and high antioxidant activity.
出处
《核农学报》
CAS
CSCD
北大核心
2017年第2期298-306,共9页
Journal of Nuclear Agricultural Sciences
基金
中国农业科学院创新工程项目(1251416101535)
关键词
飞行时间质谱
宁夏枸杞
多酚
抗氧化
CACO-2细胞
time of flight mass spectrometry,Lycium barbarum L. ,polyphenol, antioxidant activity, Caco - 2
