摘要
目的:采用HPLC法建立紫穗槐根的指纹图谱,并建立同时测定紫穗槐根中毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素含量的方法。方法:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.2%磷酸溶液(B)为流动相,梯度洗脱;检测波长为260 nm。对采集的10批紫穗槐根进行测定,运用"中药色谱指纹图谱相似度评价软件"(2004A)版进行相似度评价,运用SPSS软件进行聚类分析。结果:建立了紫穗槐根的HPLC指纹图谱及4种成分的含量测定方法。10批紫穗槐根的HPLC指纹图谱共标定16个共有峰,相似度均在0.90以上。结论:该方法建立的紫穗槐根HPLC指纹图谱特征性强、重复性好,为紫穗槐根的鉴定及质量控制提供了参考。
Objective： To establish HPLC fingerprint in the root of Amorpha fruticosa,and simultaneously to determine the content of calycosin-7-O-β-D-glucopyranoside,ononin,calycosin,formononetin. Methods： The analytical column was Diamonsil C18（ 250 mm ×4. 6 mm,5 μm）. The mobile phase was acetonitrile（ A）-water（ B）（ containing 0. 2% phosphoric acid） in gradient elution,and the detection wavelength was set at 260 nm.＂Chromatographic fingerprint similarity evaluation software＂version（ 2004A） was used to evaluate similarity for the ten batches medicinal materials,and SPSS software was used for cluster analysis. Results： The HPLC fingerprint of the root of Amorpha fruticosa was established with good separation,and four chemical compositions were determinated. 16 common peaks were defined in the HPLC fingerprint among the 10 batches of the root of Amorpa fruticosa. The similarity among them was more than0. 90. Conclusion： This analytical method has strong features,with a good repeatability and the method is simple,which can be used efficiently in the quality control in the root of Amorpha fruticosa.
出处
《中药材》
CAS
CSCD
北大核心
2016年第9期2041-2045,共5页
Journal of Chinese Medicinal Materials
基金
吉林省中医药科技项目(2014-Q40)
关键词
紫穗槐
指纹图谱
含量测定
Amorpha fruticosa L.
HPLC fingerprint
Determination
