摘要
目的研究Notch1基因在不同宫颈组织中的表达,探讨其在宫颈癌发生发展中的意义。方法分别采用免疫组化和荧光实时定量PCR检测27例子宫颈癌、33例宫颈上皮内瘤变和19例正常宫颈组织中Notch1基因的表达情况。结果免疫组化检测Nocth1蛋白在宫颈癌、宫颈上皮内瘤变组及正常宫颈癌组的表达率分别为63.0%、45.5%、15.8%,组间比较差异均有统计学意义(P〈0.05);荧光实时定量PCR检测宫颈癌、宫颈上皮内瘤变组织及正常宫颈组织中NotchlmRNA含量分别为(5.10±1.91)、(3.24±0.69)、(2.07±0.40),组间比较差异均有统计学意义(P〈0.05)。宫颈癌NotchlmRNA的高表达与患者年龄、组织学分级、临床分期差异均无统计学意义(P〈0.05)。结论Notch1mRNA的高表达与宫颈组织的恶变演进有关,在子宫颈癌的发生、发展上起重要作用。
Objective This study aimed to determine the expression profiles of Notchl gene in different tissues of the cervix, including cervical carcinoma, cervical intraepithelial neoplasia and normal endometrium, and to examine explore its significance in cancer development occurs. Methods Immunohistochemistry and fluorescence quantitative PCR ( RT-PCR ) were used to detect the expression of Notchl genes in 27 cases of cervical cancer, 33 cases of cervical intraepithelial neoplasia ( CIN ) and 19 cases of normal cervical tissue. Results Immunohistocbemistry showed the expression of Nocthl protein in cervical cancer, cervical intraepitbelial neoplasia and normal cervical group was respectively 63.0%, 45.5%, 15.8%, the difference between the two groups were statistically significant (P〈0.05) ; real-time PCR detection the Notchl mRNA contents of cervical cancer, cervical intraepithelial neoplasia and normal cervical tissues were 5.10 ± 1.91, 3.24 ± 0.69, 2.07 ± 0.40, differences between groups were statistically significant (P〈0105) . The relation of the high expression of NotchlmRNA is related to the age, histological grade, clinical stage ( P〈0.05 ) . Conclusions The high expression of NotchlmRNA in cervical tissue is related to the occurrence of the malignant evolution and play an important role in the development of cervical cancer.
出处
《浙江临床医学》
2017年第2期231-232,235,共3页
Zhejiang Clinical Medical Journal
基金
浙江省人口及计划生育项目[(2011)50号]
关键词
NOTCH1
宫颈癌
荧光实时定量PCR
免疫组化
Notchl Cervical carcinoma Real-time quantitatie polymerase chain reaction ( RT-PCR ) Immunohistochemistry