摘要
目的利用插入-缺失多态性(insertion-deletion,InDel)遗传标记,建立一套可用于鉴定中国人群的法医DNA复合扩增体系。方法利用db SNP数据库,筛选出30个在中国人群中有高度遗传多态性的InDel位点,建立复合多重PCR扩增体系,并对汉族、哈萨克族、傣族、苗族与瑶族进行遗传多态性调查。结果成功建立了一套包含30个InDel位点与1个性别鉴定基因座、共31个遗传标记的复合多重PCR扩增体系。在5个民族的遗传多态性调查中,累积个体识别率分别为0.999999999957、0.999999999990、0.999999999974、0.999999999875及0.999999999966,具有较高的遗传多态性;群体间FST值均小于0.0448,在群体间差异很小。结论本体系可用于中国人群的法医DNA个体识别。
Objective To establish a multiplex PCR system by the insertion-deletion polymorphic（InDel） genetic markers for forensic DNA identification of Chinese population. Methods 30 highly-polymorphic InDel markers were selected by resort of the db SNP database for Chinese population. The multiplex PCR system was developed by a five-fluorescence dye labeling system. The InDel polymorphism in the ethnic populations of Han, Kazak, Dai, Miao and Yao was investigated and its genetic characteristics determined. Results A multiplex PCR system, containing 30 highly polymorphic InDel markers and an Amelogenin gender marker, has been successfully established. The cumulative discrimination power（CDP） of the 30 InDel markers is respective of 0.999999999957, 0.99999999999, 0.999999999974, 0.999999999875 and 0.999999999966 for the Han, Kazak, Dai, Miao and Yao ethnic population while the pairwise FST estimates between every two populations are less than 0.0448. Genetic survey showed that the 30 InDel markers are of highly polymorphism and small differences between ethnic groups. Conclusion The established multiplex PCR system is able to be used for forensic DNA identification of Chinese population.
作者
王玮
赵蕾
江丽
刘京
黄美莎
李冉冉
刘佳佳
马泉
王英元
李彩霞
WANG Wei ZHAO Lei JIANG Liz LIU Jing HUANG Meisha LI Ranran LIU Jiajial MA Quan WANG Yingyuan LI Caixia(Shanxi Medical University, Taiyuan 030001, China Institute of Forensic Science of Ministry of Public Security ＆ Beijing Engineering Research Center for Evidence Examination of Crime Scene, Beijing 100038, China)
出处
《刑事技术》
2017年第1期1-8,共8页
Forensic Science and Technology
基金
中央级公益性科研院所基本科研业务费专项资金项目(No.2014JB010)
关键词
法医遗传学
插入-缺失多态性
个体识别
forensic genetics
insertion-deletion（InDel） polymorphism
personal identification
