摘要
旨在评价植物乳杆菌H18对4-硝基喹啉-1-氧化物(4-NQO)基因毒性的抑制作用。利用SOS显色反应对指示菌E.coli PQ37进行验证,确定其产生β-半乳糖苷酶和碱性磷酸酶的性能;通过测定植物乳杆菌H18与4-NQO共培养体系中β-半乳糖苷酶的活性,评价植物乳杆菌H18抑制4-NQO基因毒性的能力。结果显示,一定浓度的4-NQO能够诱导E.coli PQ37表达β-半乳糖苷酶和组成型碱性磷酸酶;当4-NQO的浓度范围在0~333.33 ng/m L时,E.coli PQ37的组成型碱性磷酸酶活性保持稳定状态(11.95~14.40 U),表明选取的4-NQO浓度范围合适,可以开展后续试验;植物乳杆菌H18与3个浓度的4-NQO共培养后,均降低了共培养体系中E.coli PQ37的β-半乳糖苷酶活性,其中植物乳杆菌H18对83.33 ng/m L的4-NQO基因毒性的抑制率在3个4-NQO设定浓度中最高,为42.87%,不属于高抗突变菌株。综上表明,植物乳杆菌H18能够降低4-NQO的基因毒性。
The aim of the present study was to evaluate the inhibitory effect of Lactobacillus plantarum HI8 on genotoxicity induced by 4-NQO. The β-galactosidase and alkaline phosphatase producing feature of indicator strain E. coli PQ37 was identified by using SOS-chromotest, and the activity of [3-galactosidase in co-culture system of Lactobacillus plantarum H 18 and 4-NQO were determined. The results revealed that the expression of β-galactosidase and alkaline phosphatase was induced by a certain concentration of 4-NQO; when E. coli PQ37 was treated with 4-NQO over a range of concentrations （0-333.33 ng/mL）, the activity of alkaline phosphatase remained stable （ 11.95-14.40 U）, indicating that the selected concentration range of 4-NQO was proper for the successive test; the activity of β-galactosidase in co-culture system of LactobaciUus plantarum H18 and 3 concentrations of 4-NQO was decreased compare to the negative control; When Lactobacillus plantarum H18 co-cultured with 83.33 ng/mL 4-NQO, the highest inhibition rate （42.87%） was observed, indicating that Lactobacillus plantarum H18 was not a high-antinmtagenicity strain. In conclusion, Lactobacillus plantarum H18 shows inhibitory effect on genotoxicity induced by 4- NQO.
出处
《畜牧与饲料科学》
2017年第1期6-10,共5页
Animal Husbandry and Feed Science
基金
国家自然科学基金项目(31260566)
