摘要
目的研究玉屏风散对哮喘模型小鼠气道上皮细胞分泌的胸腺基质淋巴细胞生成素(TSLP)的抑制作用。方法 40只BALB/c小鼠随机分成4组,即空白对照组、OVA模型组、玉屏风散治疗组和地塞米松治疗组,各组采取相应干预措施。肺匀浆TSLP检测使用ELISA方法,肺组织TSLP mRNA采用qRT-PCR方法检测,TSLP在肺部的表达采用免疫组织化学方法。结果在肺匀浆中TSLP在OVA诱导的哮喘模型组并未发现增高,且各组TSLP表达差异无统计学意义(P>0.05);哮喘模型组白细胞介素-13(IL-13)和白细胞介素-17(IL-17)显著增高,而玉屏风散和地塞米松均能显著降低IL-13和IL-17水平(P<0.05,P<0.01)。TSLP mRNA在哮喘模型组与空白对照组比较表达增强(P<0.05)。给予玉屏风散和地塞米松对TSLP及其TSLP mRNA无抑制表达作用(P>0.05)。结论玉屏风散能降低哮喘模型小鼠的肺部IL-13和IL-17炎症细胞因子的水平,但对TSLP的表达没有抑制作用。
Objective To study the inhibitory effects of Yupingfeng Powder on thymic stromal lymphopoietin （TSLP） secreted in airway epithelial cells of asthma model mice. Methods Forty BALB/c mice were randomly divided into the blank eontrol group, OVA model group, Yupingfeng Powder treatment group and dexamethasone treatment group. Corresponding interventions were taken in each group. The level of TSLP in lung homogenate was detected by EL1SA, the mRNA expression of TSLP in lung tissue was determined by quantitative real-time PCR, and the expression of TSLP in lung was determined by immunohistochemistry. Results The level of TSLP in lung homogenate of OVA-induced asthma model mice was not increased compared with the control group, and there was no statistically significant difference on the level of TSLP in lung homogenate among groups（ P 〉 0.05 ）. The levels of interleukin- 13 （ IL- 13 ） and interleukin- 17 （IL-17） in the asthma model group were signifieantly increased compared with the control group, and Yupingfeng Powder and dexamethasone treatment could significantly reduce the levels of IL- 13 and IL- 17 （ P 〈 0.05, P 〈 0.01 ）. The TSLP mRNA expression in the asthma model group was up-regulated compared with the eontrel group （ P 〈 0.05 ）. Yupingfeng Powder and dexamethasone treatment showed no inhibitory effects on the TSLP level and TSLP mRNA expression （P 〉 0.05 ）. Conclusion Yupingfeng Powder can decrease the levels of IL- 13 and IL- 17 in lung of asthma model mice, but shows no inhibitory effects on the expression of TSLP.
作者
王华
王嵩
丁肖媛
陆磊
蔡红
王巧娥
沈朝斌
WANG Hua WANG Song DING Xiao-yuan LU Lei CAI Hong WANG Qiao-e SHEN Chao-bin(Department of Pediatrics, Shanghai Municipal Hospital of Integrated Chinese and Western Medicine, Shanghai, 20008)
出处
《上海中医药杂志》
2017年第2期69-72,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市中医药科研基金资助项目(2012J010A)
