摘要
为了解广东地区猪流感病毒(SIV)的流行和变异情况,本研究于2013年10月至2014年1月从广东省4个不同地区采集猪鼻拭子和肺脏病料共203份,对样品处理之后进行鸡胚分离和RT-PCR检测,从中分离得到5株SIV,对其进行病毒纯化、全基因组测序和遗传进化分析。结果表明,5株SIV分离毒株其中3株为H1N1亚型,2株为H1N2。分离毒株HA裂解位点位于PSIQSR↓GL,具有典型的低致病性流感病毒特征。HA基因序列比对结果显示,5株SIV分离毒株与A/Jiangsu/ALS1/2011(H1N1)株同源性最高,核苷酸相似性为97%~99%。遗传进化分析结果显示,5株分离毒株的PB2、PB1、PA和NP基因片段属于PDM/09分支,M基因片段以及外部基因片段HA属于欧亚类禽分支,NS基因片段属于北美三元重组分支,3株H1N1亚型的NA基因属于欧亚类禽分支,2株H1N2亚型的NA基因属于人季节性流感分支。抗原位点分析结果表明,分离毒株的抗原位点基本保守,但YJ28分离株HA1蛋白上发现3个氨基酸突变,分别是L69S、S137P、E222G。本研究通过对广东省不同地区分离到的SIV进行鉴定分析,掌握SIV的变异趋势,为猪流感的防控提供切实有效的理论依据。
To investigate the epidemic and variation of swine influenza virus （SIV） in Guangdong province,a total of 203 specimens included nasal swab and lung issue were collected from pigs in 4 areas of Guangdong province from Nov/2013 to Jan/2014. After specimens treatment,inoculation in 10-days-old embryonated eggs and RT-PCR test,5 SIV virus strains were isolated. The complete genes were sequenced and the genetic analysis showed that 3 virus strains were H1N1 SIV and 2 strains were H1N2 SIV. The cleavage site of these viruses located in PSIQSR GL,which was the symbol of low pathogenic influenza A/Jiangsu/ALS1/2011 （HIN1） ,the PB1,PA and NP of 5 virus strains vlru deg bel s. The genetic ana ree of nucleotide onged to PDM09 sis showed the 5 isolates were close to entical were between 97%-99%. PB2, ranch, M gene and external gene HA belonged to Euro strain and NA of analysis demonst strain had 3 sites pc-Asia-avian-like strain,NA of 3 H1N1 stains was from Europe-Asia-avian-like 2 H1N2 strains was from human seasonal influenza. The result of aantigenic site rated that isolated virus antigenic site were generally conserved. However,YJ28 of amino acid mutated in HA1 protein,which was L69S,S137P and E222G,re spectively. This study isolated and identified SIVs from Guangdong province, understanding the mutation trend of SIV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第2期266-271,共6页
Chinese Journal of Veterinary Science
基金
现代农业产业技术体系资助项目(CARS-36)
关键词
广东地区
猪流感病毒
分离鉴定
相似性
遗传进化分析
抗原位点
Guangdong area
swine influenza virus
separation identification
similarity
analysis of genetic evolution
antigen sites
