摘要
目的:检测人质子感知受体G蛋白偶联受体2A(G2A)和卵巢癌G蛋白偶联受体1(OGR1)在低氧性肺动脉高压(HPH)患者外周血细胞中的变化。方法:研究对象选取31例HPH患者为低氧性肺动脉高压组(HPH组),男性16例,女性15例,年龄(65.19±5.86)岁。同时符合中华医学会呼吸病学分会慢性阻塞性肺疾病学组诊断标准和呼吸衰竭诊断标准,选取30例健康体检者为正常组(NC组),男15例,女15例,年龄(63.47±6.16)岁。心脏彩超计算HPH组肺动脉压力、进行血气分析和肺功能检测,采集外周血检测G2A、OGR1基因mRNA表达水平、血清肿瘤坏死因子α(TNF-α)水平。结果:HPH组Pa CO2较NC组明显增高(P<0.05),1 s用力呼气容积占预计值的百分比(FEV1pro%)和1 s用力呼气容积与用力肺活量比值(FEV1/FVC)明显低于NC组(P<0.05)。HPH组外周血中G2A mRNA及TNF-α含量明显高于NC组(P<0.05)。OGR1 mRNA与NC组无差别。HPH组G2A mRNA及TNF-α表达与肺动脉压力呈显著正相关。结论:肺动脉高压患者外周血细胞中质子感知受体G2A表达增加,TNF-α水平增加,G2A的表达和TNF-α水平与肺动脉压力呈明显正相关。
Objective： To detect the expression changes of proton-sensing receptor G2 accumulation（ G2A） and ovarian cancer G protein-coupled receptor 1（ OGR1） in human peripheral blood cells in hypoxic pulmonary hypertension patients（ HPH）. Methods：Thirty-one patients with HPH were enrolled for HPH group（ 16 men and 15 women,age：（ 65. 19 ± 5. 86） and thirty healthy persons were enrolled for the control group（ NC group）. The peripheral blood samples were collected and the m RNA expressions of G2 A and OGR1 were determined by using real-time fluorescent quantitative PCR. The serum levels of tumor necrosis factor-α（ TNF-α） were detected by using enzyme-linked immunosorbent assay（ ELISA）. Results： Pa CO2 was increased significantly in HPH group than that of the NC group（ P〈0. 05）. Forced expiratory volume in 1 sencond（ FEV1） pro% and FEV1 / forced vital capacity（ FVC） in HPH group were significant lower than those of the NC group（ P〈0. 05）. The expressions of peripheral blood G2 A m RNA and TNF-α in HPH group were increased dramatically than those of the NC group（ P〈0. 05）. The expressions of OGR1 m RNA in peripheral blood had no difference between HPH group and NC group. The expressions of G2 A and TNF-α in HPH group were positively related to pulmonary artery pressure significantly. Conclusion： The expression of proton-sensing receptor G2 A and the level of TNF-α are increased in peripheral blood cells of patients with pulmonary hypertension. The expressions of TNF-α,G2 A and pulmonary artery pressure have positive correlation in the HPH group.
作者
武敏
杨敬平
徐喜媛
郭智敏
卜宝英
WU Min YANG Jlng-pmg XU Xi-yuan GUO Zhi-min BU Bao-ying(The Third Affiliated Hospital of Inner Mongolia Medical University, BaoGang Hospital, Baotou 014010, China)
出处
《中国应用生理学杂志》
CAS
CSCD
2017年第1期85-88,共4页
Chinese Journal of Applied Physiology
