摘要
目的了解近年来新疆沙门氏菌血清型分布、耐药情况及分子分型特征。方法 2015年对收集的43株沙门氏菌进行血清分型,并运用CLSI推荐的微量肉汤稀释法测定耐药性,采用脉冲场凝胶电泳法进行分子分型。结果43株沙门氏菌属于12个血清型,优势血清型为肠炎沙门氏菌和鼠伤寒沙门氏菌;发现32株多重耐药沙门氏菌,多重耐药率达74.42%,耐4种及以上抗生素27株、耐药率为62.79%,耐7种及以上抗生素15株、耐药率为37.50%;43株沙门氏菌呈现出28个不同的基因型别,常见沙门氏菌血清型有较好的成簇现象,相同血清型的基因型比较相近具有同源性,不同血清型的PFGE结果具有多态性。结论新疆沙门氏菌存在高耐药和多重耐药现象,利用PFGE分子分型方法,能更好地揭示不同来源沙门氏菌菌株之间的亲缘性和基因耐药性关系。
Objective To investigate the serotype distribution, drug resistance and molecular types of Salmonella in Xinjiang in recent years. Methods The collected 43 strains of Salmonella were serotyped in 2015, and their drug susceptibility was detected with broth microdilution method recommended by CLSI. Molecular types were identified wtih PFGE. Results Twelve serotypes were found in 43 Salmonella strains. Salmonella enteritidis and Salmonella typhimurium were the dominant serotypes. Thirty-two strains of Salmonella were multiple drug resistance, with drug resistance rate of 74.42%, including27 strains resistant to more than 4 kinds of antibiotic with resistance rate of 62.79%, 15 strains resistant to more than 7 kinds of antibiotics with the resistance rate of 37.50%. Forty-three strains of Salmonella presented 28 genotypes. The serotype of common Salmonella had superior cluster phenomenon. Genotypes of the same serotype were similar and there were homogenous strains. The PFGE result of different serotypes had polymorphism. Conclusions Salmonella in Xinjiang shows high drug-resistance and multiple drug resistance. The affinity and drug resistance of Salmonella from different sources are well revealed with PFGE method.
出处
《疾病预防控制通报》
2016年第6期9-12,49,共5页
Bulletin of Disease Control & Prevention(China)
关键词
沙门氏菌
血清型
耐药性
分子分型
Salmonella
Serotype
Drug resistance
Molecular typing
