摘要
[ Objectives ] To study the pathogenicity of rotavirns causing piglet diarrhea in Beijing area. [ Methods ] Six newborn piglets were randomly divided into two groups ： three piglets in test group and three in control group. A total of 3 mL three-generation cell virus were evenly assigned to the three test piglets by both oral mode and subcutaneous injection, and the detoxification was observed. Anal swabs were collected at the 24^th, 48^th, 72^th and 96^th hours before and after the infection respectively, virus antigens were detected by RT-PCR, detoxlllcation was observed; small intestine, liver, spleen and other tissues of the 5-d-infected piglets were collected to observe the pathological changes. [ Results ] Anal swab detection showed that rotavirus could be detected in piglets at the 12^th after being in- fected, and the situation could continue to the 5^th d. Observation on tissue sections showed that piglet small intestinal epithelial cells and surrounding villi degenerated, epithelial cells became rounded, swollen; and enlarged nuclei and irregular cell margins could be observed. Degenerated cells fell off from matrix to lumen, at- rcphy villi was covered by flat epithelial cells, a small amount of cell debris could be observed in lamina propria. [ Conclusions ] The infection severity of porcine rotavirus depends on the atrophy degree and distribution of small intestinal villi. In addition, host, environment and other relevant factors also played an important role in the pathogenesis of porcine rotavirus.
[ Objectives ] To study the pathogenicity of rotavirns causing piglet diarrhea in Beijing area. [ Methods ] Six newborn piglets were randomly divided into two groups ： three piglets in test group and three in control group. A total of 3 mL three-generation cell virus were evenly assigned to the three test piglets by both oral mode and subcutaneous injection, and the detoxification was observed. Anal swabs were collected at the 24^th, 48^th, 72^th and 96^th hours before and after the infection respectively, virus antigens were detected by RT-PCR, detoxlllcation was observed; small intestine, liver, spleen and other tissues of the 5-d-infected piglets were collected to observe the pathological changes. [ Results ] Anal swab detection showed that rotavirus could be detected in piglets at the 12^th after being in- fected, and the situation could continue to the 5^th d. Observation on tissue sections showed that piglet small intestinal epithelial cells and surrounding villi degenerated, epithelial cells became rounded, swollen; and enlarged nuclei and irregular cell margins could be observed. Degenerated cells fell off from matrix to lumen, at- rcphy villi was covered by flat epithelial cells, a small amount of cell debris could be observed in lamina propria. [ Conclusions ] The infection severity of porcine rotavirus depends on the atrophy degree and distribution of small intestinal villi. In addition, host, environment and other relevant factors also played an important role in the pathogenesis of porcine rotavirus.
基金
Supported by Beijing"Vegetable Basket"Science and Technology ability upgrading project&Beijing Agricultural Vocational College Project(XY-YF-16-24)
