摘要
为研究牛磺胆酸(Taurocholic Acid,TCA)对NR8383细胞分泌的白介素-1β(interleukin-1β,IL-1β)的影响,通过实时荧光定量PCR、酶联免疫吸附法检测了TCA对脂多糖诱导的NR8383细胞分泌IL-1βmRNA和蛋白含量的影响。结果显示,脂多糖(LPS)极显著增加IL-1βmRNA和蛋白含量(P<0.01);1 000μmol/L TCA可降低LPS诱导的NR8383细胞分泌IL-1βmRNA和蛋白含量;500μmol/L、100μmol/L的TCA可极显著降低LPS诱导的NR8383细胞分泌IL-1βmRNA和蛋白含量(P<0.01)。结论:TCA可降低经LPS诱导的NR8383细胞分泌IL-1β基因、蛋白水平含量,随着剂量的降低呈现剂量依赖性的抑制作用,表明TCA具有抗炎免疫调节作用。
In this study we investigated the comprehensive pharmacological effects of taurocholic acids（ TCA,a kind of natural bioactive substance of animal bile acids） on the production of IL-1β in NR8383 cells. The results showed that TCA had a suppressive effect on LPS induced NR8383 cells by analyzing IL-1 mRNA and protein levels using the q PCR and ELISA. The level of IL-1β mRNA and protein induced by LPS was significant（ P 〈 0. 01）. TCA at a concentration of 1000,500 or 100 μM significantly decreased LPS-induced production of IL-1β in NR8383 cells（ P 〈 0. 01）. Our findings suggest that TCA has an anti-inflammatory immune regulating effect.
作者
王彩云
张召议
张子英
关红
李培锋
WANG Cai-yun ZHANG Zhao-yi ZHANG Zi-ying GUAN Hong LI Pei-feng(College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018 ,China Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Hohhot 010018, China College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, China)
出处
《中国兽医杂志》
CAS
北大核心
2016年第12期21-23,27,共4页
Chinese Journal of Veterinary Medicine
基金
内蒙古自治区高校自然科学重点项目(NJZZ049)
国家自然科学基金项目(31160487)
