摘要
以桂枝等18种中药材水提液为试验材料,检测水提液为0.5 mg·μL-1生药浓度时对5-脂肪氧化酶(5-LOX)的抑制率。结果表明,18种中药材水提液的抑制活性范围,即抑制率在1.13%~96.06%之间。以迷迭香水提液为例,4次平行试验证明,该方法的RSD(相对标准偏差)为1.17%(<2.0%)。以厚朴水提液为例,通过倍比稀释试验,表明在一定浓度范围内,水提液浓度的lg值和抑制率间存在良好的线性关系,决定系数R2值为0.958 7,厚朴水提液的抑制活性IC50值为22.38μg·μL-1。结果表明,不同的中药材对5-LOX的抑制率变化较大。该试验方法具有简单、直接、快速、结果稳定且花费较低等优点,并可精准定量中药液对5-LOX活性的抑制浓度。
Eighteen aqueous extracts of medicinal plants containing crude drug 0. 5 mg·μL-1were prepared and their inhibition rates to 5-lipoxygenase（5-LOX） activity were measured. The results showed that inhibition rate of these extracts ranged from 1. 13% to 96. 06%. In the test of Rosmarinus officinalis extract,the RSD（relative standard deviation） of this method was 1. 17%（ 〈2. 0%） out of 4 independent parallel tests. In the test of Magnolia officinali extract,by multiple dilution it showed that there was a good linear relationship between the lg value of extract concentration and the inhibition rate in certain concentration range,with the determination coefficient of 0. 958 7. The IC50 of inhibitory activity of Magnolia officinalis extract was 22. 38 μg·μL^-1. These results showed that inhibitory activity of various aqueous extracts to 5-LOX activity were different. This test method was demonstrated to have many merits such as easy and fast in operation,direct and stable in result,low cost,and able to quantify the accurate inhibition concentration of samples on the activity of 5-LOX.
出处
《浙江农业学报》
CSCD
北大核心
2017年第1期31-36,共6页
Acta Agriculturae Zhejiangensis
基金
国家现代农业产业技术体系(CARS-36)
农业部公益性行业科研专项(201403047)
"十二五"农村领域国家科技计划课题(2012BAD39B00)
关键词
脂肪氧化酶
中药材水提液
抑制活性
lipoxygenase
traditional Chinese medicine aqueous extracts
inhibitory activity
