摘要
目的分析浙江省宁波市2003-2014年肠炎沙门菌脉冲场凝胶电泳(PFGE)分型及耐药特征。方法采用Pulse Net China网络实验室的标准方法,对2003-2014年间分离的68株肠炎沙门菌进行XbaⅠ和BlnⅠ酶切分型,使用Bio Numerics软件对菌株的PFGE图谱进行聚类分析;使用纸片扩散法检测菌株对10种抗生素的耐药性。结果 68株肠炎沙门菌经XbaⅠ酶切分为8个PFGE带型。其中JEGX01.NB0001型为最优势型别,共53株,带型相似度90%以上的菌株占95.6%(65/68),2003年和2013年超过80例以上的食源性暴发案例的肠炎菌株经双酶切聚类证实存在100%的同源性。所有肠炎沙门菌对抗生素耐药率为氨苄西林23.5%、强力霉素4.4%、氨曲南、头孢吡肟和头孢噻肟均为2.9%、氯霉素和复方新诺明均为1.5%,对庆大霉素、环丙沙星、亚胺培南均敏感,发现2株多重耐药菌。结论宁波市散发和暴发的肠炎沙门菌分离株之间的分子带型差异度小,引发食源性暴发的流行株克隆之间存在流行病学关联。目前肠炎沙门菌对抗生素的耐药率处于较低水平,仍需加强对多重耐药株的监控。
Objective To analyze the Pulsed-field gel electrophoresis（PFGE） subtype and antibiotic resistance of Salmonella enteritidis strains isolated in Ningbo,Zhejiang province,during 2003-2014,to understand the circulating features of this pathogen,and provide for the prevention and control of foodborne diseases caused by S.enteritidis.Methods PFGE patterns of 68 S.enteritidis strains were obtained following the Pulse Net China protocol.The image data were analyzed by Bio Numerics（Ver.6.6）.The susceptibility of the isolates to 10 antibiotics was detected by disk diffusion method.Results Overall,68 S.enteritidis strains were subtyped into 8 PFGE patterns by XbaⅠ digestion,and 95.6%（65/68） of isolates shared ≥90% similarity.The predominant pattern JEGX01.NB0001 included 53 strains（77.9%）.This pattern S.enteritidis caused 2 serious food poisoning outbreaks in 2003 and 2013 respectively,and in each outbreak,more than 80 cases were reported.The overall resistant rate was 23.5% to ampicillin,4.4% to doxycycline,2.9% to aztreonam,cefepime and cefotaxime,and 1.5% to chloramphenicol and trimethoprim-sulfamethoxazole.All the strains were sensitive to gentamicin,ciprofloxacin and imipenem.There were 2 multidrug resistant strains.Conclusion The study has showed a clustering trend in PFGE patterns of S.enteritidis in Ningbo.Epidemiological links were found among the clone strains causing outbreaks.Lowantibiotic resistance was found through antibiotic susceptibility test,but the surveillance for multidrug resistance still needs to be strengthened.
作者
杨元斌
宋启发
闫鹏
高红
叶硕
YANG Yuan-bin SONG Qi-fa YAN Peng GAO Hong YE Shuo.(Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, Zhejiang, China)
出处
《疾病监测》
CAS
2016年第12期1007-1011,共5页
Disease Surveillance
基金
浙江省医药卫生科技项目(No.2016KYB274)~~
关键词
肠炎沙门菌
脉冲场凝胶电泳
聚类分析
优势基因型
耐药
Salmonella enteritidis
Pulsed-field gel electrophoresis
Cluster analysis
Predominant genotype
Antibiotic resistance
