摘要
目的:本文建立了近红外光谱吲哚菁绿(Indocyanine Green,ICG)成像技术平台,通过类风湿关节炎TNF-Tg小鼠模型和手术诱导骨性关节炎小鼠模型,验证了技术平台检测淋巴回流功能的成像质量与稳定性,为筛选中药复方与中药单体调控淋巴回流功能防治关节炎的动物体内研究提供技术平台。方法:采用TNF-tg小鼠和手术诱导骨性关节炎小鼠模型,采用近红外光谱ICG成像系统检测类风湿关节炎小鼠模型的下肢淋巴回流功能,手术诱导骨性关节炎小鼠模型的膝关节淋巴回流功能。结果:淋巴回流功能检测显示,与Control组相比,TNF-Tg组的下肢淋巴回流清除率明显下降;Control组小鼠下肢淋巴管收缩脉冲值为1.12±0.27个/min,与之相比,TNF-Tg组检测不到明显的收缩脉冲值;与Sham组相比,OA组的膝关节淋巴回流清除率明显下降。结论:本文建立的近红外光谱-ICG成像系统可以为评价动物体内淋巴回流功能实验研究,筛选治疗关节炎有效的中药复方,小分子化合物提供良好的工作平台。
This study aimed at establishing near infrared（NIR）-indocyanine green（ICG） imaging system to validate the imaging quality and stability of the imaging system by testing the ICG clearance of the footpads of mice with rheumatoid arthritis（RA） and knee joints of those with osteoarthritis（OA）. The joint clearances of TNF-Tg mice and surgery-induced knee osteoarthritis（KOA） mice were detected using the imaging system in this study. As a result, it was found that the ICG clearance of the footpads of TNF-Tg mice decreased（36.80%±6.70%） compared with those of the control group（77.4%±3.46%, P 〈 0.05）. The pulse number of lower limb lymphatic vessels in TNF-Tg mice was unavailable to detect, while the pulse number of the control group was 1.12±0.27 pulses per minute. The ICG clearance in the knee joints of the KOA mice alse decreased（47.84%±5.21%） compared with those of the sham group（71.60%±6.38%, P 〈 0.05）. In conclusion, the NIR-ICG imaging system provided desirable technology platform for evaluating in vivo lymphatic draining function and screening formula or monomer of traditional Chinese medicine as the administration of arthritis.
作者
徐浩
王腾腾
齐晓凤
王琼
张利
邢联平
施杞
王拥军
梁倩倩
Xu Hao Wang Tengteng Qi Xiaofeng Wang Qiong Zhang Li Xing Lianping Shi Qi Wang Yongjun Liang Qianqian(Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China Institute of Spine Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China University of Rochester Medical Center, New York 14623, U.S.A. School of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China)
出处
《世界科学技术-中医药现代化》
2016年第11期1862-1868,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委重点项目(81330085):痰瘀型类风湿性关节炎与淋巴关系的基础研究
负责人:施杞
国家自然科学基金委重大国际合作项目(81220108027):益气祛湿法调控淋巴系统对炎症性关节炎的影响
负责人:王拥军
国家自然科学基金委青年基金项目(81403418):TNF-α介导NF-κB信号通路活化在骨性关节炎形成中的作用及蠲痹汤防治的机制研究
负责人:徐浩
教育部高等学校全国优秀博士学位论文作者专项资金资助项目(201276):负责人:梁倩倩
上海市龙华医院龙医创新团队(LYCX-01):祛瘀化瘀防治颈痛和炎性关节炎的基础研究
负责人:梁倩倩
上海市长宁区卫生与计划生育委员会"光华卓越PI工程"项目(2016-01)
负责人:施杞
筋骨理论与治法教育部重点实验室
负责人:王拥军
关键词
近红外光谱成像
吲哚菁绿
淋巴回流系统
关节炎
Near infrared imaging
indocyanine green
arthritis
lymphatic drainage function
