摘要
目的建立快速、准确的HPLC-Q-TOF法测定降糖类中成药及保健食品中14种非法添加药物。方法样品采用50%乙腈提取,色谱柱为Thermo syncronis C18(150mm×4.6mm,5μm),流动相:0.1%甲酸-乙腈梯度洗脱,流速:0.3ml·min^(-1),柱温:30℃。采用ESI源,正离子扫描模式。结果 14种非法添加化合物在0.01~1μg·ml^(-1)范围内峰面积与浓度均呈良好的线性关系,r>0.998,回收率在78.0%~98.6%,RSD在2.3%~8.3%,检出限均在0.03~0.10μg·g^(-1)范围内。结论本方法快速准确,灵敏度高,能够同时满足对降糖类中成药及保健食品中14种非法添加药物的定性定量分析。
Objective To establish a quick,accurate method for determination of fourteen types of illegally added antidiabetics in traditional Chinese medicines（TCM）and health care products by performance liquid chromatography-quadrupole/time of flight mass spectrometer.Methods The samples were extracted with 50% acetonitrile,and separated on a Thermo syncronis-C18column（150mm×4.6mm,5μm）with 0.1%formic acid solution-acetonitrile as the mobile phase by gradient elution at a flow rate of 0.3ml·min^（-1）.The column temperature was 30 ℃.The positive mode with ESI＋source was used in the MS detection.Results The linear relationship of all the 14 antidiabetics was good in the range of 0.01-1μg·ml^（-1） with the correlation coefficient greater than 0.998.The recovery ranged from 78.0% to 98.6% with the relative standard deviations at 2.3%-8.3%.The limits of quantitation ranged from 0.03 to 0.10μg·g^（-1）.Conclusion This method is quick,accurate and highly sensitive,which can be used for the determination of 14 illegally added antidiabetics in TCM and health care products.
作者
姜春来
邢俊波
靳守东
JIANG Chun-lai XING Jun-bo JIN Shou-dong(Institute for Drug and Instrument Control of Health Dept GLD of PLA,Beijing 100166,China)
出处
《解放军药学学报》
CAS
CSCD
2016年第6期514-517,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家重大科学仪器设备开发专项中北京市基药
军队特需药等综合信息入库
快检技术应用研究
No.2012YQ18013208
关键词
HPLC-Q-TOF
非法添加
中成药
保健食品
HPLC-Q-TOF
illegally added
traditional Chinese medicines
health care products
