摘要
目的 探讨心脑舒通胶囊对异丙肾上腺素介导的心肌缺血的保护作用与其抑制NADPH氧化酶Nox2和Nox4的关系.方法 观察心脑舒通胶囊（XNST capsule）对异丙肾上腺素诱导的心肌缺血大鼠心电图,血清乳酸脱氢酶（LDH）、磷酸肌酸激酶（CK）、超氧化物歧化酶（SOD）、丙二醛（MDA）、左心室NADPH氧化酶Nox2和Nox4表达水平的影响.结果 同对照组比较,异丙肾上腺素（ISO）诱导的模型组大鼠心电图ST位移值（0.27±0.05比0.01±0.00,P＜0.05）和N-ST（9.63±0.37比0.47±0.08,P＜0.05）显著增加,血清中LDH[（1356.0±292.8）U/L比（640.0±145.3）U/L,P＜0.05）]、CK[（978.0±223.5）U/L比（470.0±113.7）U/L,P＜0.05]和MDA[（9.06±2.17）μmol/L比（5.40±1.78）μmol/L,P＜0.05]的蛋白水平增高,而血清中SOD蛋白水平明显减少[（71.0±12.8）U/ml比（107.0±16.7）U/ml,P＜0.05].与ISO模型组比较,心脑舒通胶囊治疗显著降低大鼠心电图ST位移值[0.16±0.04（低剂量组）比0.27±0.05,P＜0.05;0.13±0.03（高剂量组）比0.27±0.05,P＜0.05]和N-ST[4.70±0.42（低剂量组）比9.63±0.37,P＜0.05;3.83±0.28（高剂量组）比9.63±0.37,P＜0.05]以及血清中LDH[（938.0±275.9）U/L（低剂量组）比（1356.0±292.8）U/L,P＜0.05;（889.0±125.6）U/L（高剂量组）比（1356.0±292.8）U/L]、CK[（607.0±137.2）U/L（低剂量组）比（978.0±223.5）U/L,P＜0.05;（579.0±125.6）U/L（高剂量组）比（978.0±223.5）U/L,P＜0.05]和MDA[（6.60±1.92） μmol/L（低剂量组）比（9.06±2.17）μmol/L,P＜0.05;（6.20±1.63） μmol/L（高剂量组）比（9.06±2.17）μmol/L,P＜0.05]的蛋白表达水平,而心脑舒通胶囊治疗后大鼠血清中SOD蛋白水平升高[（89.0±13.9）U/ml（低剂量组）比（71.0±12.8）U/ml,P＜0.05;（94.0±15.3）U/ml（高剂量组）比（71.0±12.8）U/ml,P＜0.05].此外,心脑舒通胶囊可显著降低异丙肾上腺素诱导的心肌缺血大鼠左心室中NADPH氧化酶Nox2和Nox4�
Objective XNST capsule could alleviate cardiac ischemia, however, little is known about the mechanisms. The aim of this research is to explore whether NADPH oxidase Nox2 or Nox4 is involved in the pro- tective action of XNST capsule or not. Methods Myocardial ischemia model induced by isoprenaline was used to test the effect of XNST capsule on electrocardiogram, the concentration of LDH, CK, SOD and MDA in serum, and the expression of NADPH oxidase Nox2 or Nox4 in left ventricular tissues. Results Compared with control group, ST displacement （0.01±0.00 vs 0.27±0.05, P〈0.05） and N-ST （0.47±0.08 vs 9.63±0.37, P〈0.05） of electrocardiogram were significantly increased in isoproterenol-induced rats, and the protein levels of LDH [（640.0±145.3）U/L vs （1356.0±292.8）U/L, P〈0.05], CK [（470.0±113.7）U/L vs （978.0±223.5）U/L, P〈 0.05 ] and MDA [ （ 5.40± 1.78 ）μmol/L vs （9.06±2.17）lxmol/L, P〈0.05 ] in plasma were remarkably elevated in these rats, whereas SOD protein level in plasma of isoproterenol-induced rats was notably decreased [ （107.0± 16.7）U/ml vs （71.0±12.8）U/ml, P〈0.05 ]. Compared with ISO group, treating with XNST capsule significantly reduced ST displacement[0.27±0.05 vs 0.16±0.04（low dose group）, P〈0.05, 0.27±0.05 vs 0.13±0.03（high dose group ）, P〈0.05 ] and N-ST] （9.63±0.37 vs 4.70±0.42（low dose group）, P〈0.05, 9.63±0.37 vs 3.83±0.28（high dose group）, P〈0.05 ] of electrocardiogram, and the plasma concentration in LDH[ （1356.0±292.8）U/L vs （938.0± 275.9）U/L （low dose group）, P〈0.05, （1356.0±292.8）U/L vs （889.0±125.6）U/L （high dose group）], CK [（978.0±223.5）U/L vs （607.0±137.2）U/L（low dose group）, P〈0.05, （978.0±223.5）U/L vs （579.0±125.6）U/L （high dose group）, P〈0.05] and MDA[（9.06±2.17）μmol/L vs （6.60±l.92）μmol/L（low dose group）, P〈0.05, （9.06±2.17）μmol/L vs （6.20± 1.63 ）μmol/L （high dose group ）, P〈0.051, whereas there
作者
陈春
戴海鹰
CHEN Chun DAI Hai-ying.(Cardiovascular Department of Changsha Central Hospital, Changsha 410000, China)
出处
《中国心血管病研究》
CAS
2016年第11期1048-1051,共4页
Chinese Journal of Cardiovascular Research
