摘要
本文采用气相质谱联用技术对三株海水微藻—三角褐指藻(Phaeodactylum tricornutum,CCMM2012)、小球藻(Chlorella vulgaris,CCMM4004)、微拟球藻(Nannochloropsis sp.,CCMM6004),和一株淡水微藻—栅藻(Scenedesmus quadricauda,CCMM4002)在不同温度下的生长及油脂变化规律进行研究。结果表明:三角褐指藻、小球藻和栅藻在20℃下的油脂产率最大,分别为2.94、5.79和5.83mg/L/d,它们在20℃的培养条件下比较适合应用于生物能源开发;而微拟球藻在25℃下油脂产率最大,达6.45mg/L/d,因此此温度下更适合用于生物能源开发。三角褐指藻在15℃条件下DHA和EPA含量最多,为16.47%,而微拟球藻在25℃下DHA和EPA最多,为22.74%;因此三角褐指藻和微拟球藻在15℃和25℃下均比较适合应用于多不饱和脂肪酸的开发,而小球藻和栅藻的EPA和DHA含量在三个温度下普遍较少,不适合用于多不饱和脂肪酸的开发。
Growth and lipid change in three marine microalgae （Phaeodactylum tricornutum, Chlorella vulgaris, and Nannochloropsis sp.） and a freshwater microalga （Scenedesrnus quadricauda） under different temperatures（15, 20, and 25℃） were studied by GC-MS. The results show that the lipid production in P tricornutum, C. vulgaris and S. quadricauda peaked at 2.94, 5.79, and 5.83mg/L/d, respectively, and these three algae shall be suitable for bioenergy preparation. For Nannochloropsis sp., the peak point of 6.45mg/L/d occurred in 25℃. The contents of EPA and DHA in P. tricornutum were the highest at 16.47% in 15℃, while for Nannochloropsis sp., they reached maximum at 22.74% in 25℃. Therefore, P tricornutum and Nannochloropsis sp. were potential appropriate microalgae for production of polyunsaturated fatty acid. However, the contents of EPA and DHA of C. vulgaris and S. quadricauda were very low under the three temperatures. Therefore, using them for polyunsaturated fatty acid would be inappropriate.
作者
赵婷
韩笑天
詹天荣
赵卫红
ZHAO Ting HAN Xiao-Tian ZHAN Tian-Rong ZHAO Wei-Hong(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China)
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2016年第6期1140-1148,共9页
Oceanologia Et Limnologia Sinica
基金
海洋可再生能源专项
GHME2001SW02号
国家自然科学基金
41276118号
国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金
MBSMAT-2016-02
关键词
温度
微藻
油脂
脂肪酸
temperature
microalgae
lipid
fatty acid
