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长顺绿壳蛋鸡PRL基因的克隆及序列分析

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摘要 采用特异性引物PCR技术获得长顺绿壳蛋鸡PRL基因,将其克隆至p GM-T载体,并对该序列进行测序分析。结果表明,长顺绿壳蛋鸡PRL基因全序列长度为690 bp,包含5个外显子和4个内含子,编码229个氨基酸。将长顺绿壳蛋鸡PRL基因与Gen Bank已公布的鸡、鸭、鹅、鱼等动物的PRL基因序列进行同源性比较,发现亲缘关系越远则同源性越低。
出处 《江苏农业科学》 北大核心 2016年第12期82-85,共4页 Jiangsu Agricultural Sciences
基金 贵州省科学技术基金(编号:黔科合J字[2011]2174) 贵州省农业科学院研究生创新基金(编号:黔农科合[创新基金]2010011) 贵州省农业科学院专项(编号:黔农科院院专项[2014]006号) 贵州省动植物育种专项(编号:黔农育专字[2011]027)
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