摘要
利用多重SSR-PCR荧光标记检测技术,对本单位自育和引进的259份玉米自交系进行遗传多样性和亲缘关系分析,并以11个归属于不同杂种优势群的代表自交系为参照进行群体结构分析。结果显示,50对SSR引物共检测到214个等位基因变异,基因多态性指数的平均值为0.50,每个SSR标记的多态性信息量(PIC)平均值为0.44。用UPGMA方法将270份自交系划分成3大类群,进一步进行数值化杂种优势群分析,以明确所用种质资源的杂优类群利用方向,为玉米自交系的有效利用和品种的选育提供依据。
Using automatic DNA analyzer multiple SSR-PCR fluorescent marker detection technology, we analyzed the genetic diversity of 259 maize germplasms and 11 kind of inbred lines through core 50 standard test simple sequence repeat loci which cover whole genome. Among 50 SSRs, the total allele No. was 214 for 270 inbred lines. The average of the gene diversity and PIC was 0. 50 and 0. 44, respectively. The 270 lines could be divided into 3 groups by UPGMA clustering method. Furthermore, we analysis the numerical value of heterosis group, in order to make clear the application of germplasm resources. The results in this study could provide the genetic basis for improving the efficiency of hybrid breeding.
作者
张全芳
梁水美
李燕
刘艳艳
范阳阳
郭庆法
鲁守平
步迅
ZHANG Quan-fang LIANG Shui-mei LI Yan LIU Yan-yan FAN Yang-yang GUO Qing-fa LU Shou-ping BU Xun(lBio-Tech Research Center, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100 Maize Research Institute, Shandong Academy of Agricultural Sciences/ National Engineering Laboratory of Wheat and Maize/State Key Laboratory of the Ministry of Agriculture on Corn Biological and Genetic Breeding in the Northern of Hnang Huai Plain, Jinan 250100)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2017年第1期19-31,共13页
Journal of Plant Genetic Resources
基金
国家玉米产业体系项目(CARS-02-06)
山东省农业良种工程项目(2014)
公益性行业(农业)科研专项经费项目(201303002)
山东省农业科学院农业科技创新工程(CXGC2016A11)
山东省中青年科学家科研奖励基金项目(BS2013SW003)
关键词
玉米自交系
分子标记
遗传多样性
maize （ Zea mays L. ） germplasms
SSR-PCR fluorescent marker
genetic diversity
