摘要
目的探讨胰腺癌进展过程中，免疫系统和肿瘤微环境中的T细胞亚群是否会随肿瘤进展而改变。方法在小鼠胰腺癌模型成瘤后第1、2、3周时，采用流式细胞术检测外周血、脾脏和肿瘤组织中CD4＋、CD8＋和CD4＋叉状头/翅膀状螺旋转录因子（Foxp3）＋细胞占CD3＋ T淋巴细胞的比例。结果肿瘤组织中，CD4＋细胞比例依次为（20.9±3.1）%、（10.1±4.9）%、（11.4±9.9）%；CD8＋细胞比例依次为（40.6±4.9）%、（41.6±3.2）%、（51.4±11.9）%、CD4＋Foxp3＋细胞比例依次为（2.34±1.69）%、（3.34±1.83）%、（1.36±0.58）%，三类细胞变化差异均有统计学意义（P＝0.003、0.026、0.033）；脾脏中，CD4＋细胞比例依次为（39.5±4.4）%、（26.5±4.8）%、（31.8±9.4）%；CD4＋Foxp3＋细胞比例依次为（4.40±0.33）%、（6.50±2.07）%、（4.97±2.32）%，其变化差异有统计学意义（P＝0.012、0.005），CD8＋细胞的变化差异无统计学意义；外周血中，CD4＋、CD8＋和CD4＋Foxp3＋细胞比例的变化差异均无统计学意义。结论小鼠胰腺癌模型中T细胞亚群的分布会随肿瘤进展发生动态变化，且CD4＋、CD8＋细胞与CD4＋Foxp3＋细胞的变化趋势相反。由于各细胞亚群的变化模式较为复杂，仍需进一步研究来探明其机制。
Objective To investigate whether the distribution of T lymphocyte subsets in the immune organs and tumor microenvironment will change or not during the progression of tumor.Methods In the 1st, 2nd, 3rd week after the tumor has established, the proportion of CD4＋ , CD8＋ and CD4＋ forkhead box P3 （Foxp3）＋ cells of CD3＋ T lymphocytes in peripheral blood, spleen and tumor were measured by flow cytometer respectively.Results In tumor tissues, the proportions of CD4＋ cells were （20.9±3.1）%, （10.1±4.9）%, （11.4±9.9）%, the proportions of CD8＋ cells were （40.6±4.9）%, （41.6±3.2）%, （51.4±11.9）%, the proportions of CD4＋ Foxp3＋ cells were （2.34±1.69）%, （3.34±1.83）%, （1.36±0.58）%, all changes had statistically significance （P=0.003, 0.026, 0.033）; in spleen, the proportions of CD4＋ cells were （39.5±4.4）%, （26.5±4.8）%, （31.8±9.4）%, the proportions of CD4＋ Foxp3＋ cells were （4.40±0.33）%, （6.50±2.07）%, （4.97±2.32）%, these changes had statistically significance （P=0.012, 0.005）, the changes of CD8＋ cells had no significance; in peripheral blood, the proportion of CD4＋ , CD8＋ and CD4＋ Foxp3＋ cells had no significant change.Conclusion In the murine pancreatic cancer model, the distribution of T cell subsets had dynamic changes during tumor progression, and the proportions of CD4＋ , CD8＋ cells had a contrast trend with the ones of CD4＋ Foxp3＋ cells. Due to the complexity of the varying model of T cell subsets, more studies should be carried out to reveal the mechanism of the anti-tumor immunity.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2017年第1期68-71,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金（81272731）
上海卫生系统先进适宜技术推广项目（2013SY053）
关键词
胰腺癌
T淋巴细胞
肿瘤浸润淋巴细胞
肿瘤进展
Pancreatic cancer
T lymphocytes
Tumor infiltrating lymphocytes
Tumor progression
