摘要
目的观察辛伐他汀对大鼠颅脑外伤后弥漫性轴索损伤的治疗效果。方法用加速-减速装置制作大鼠弥漫性轴索损伤(DAI)模型,分为安慰剂组与辛伐他汀组,口服生理盐水与辛伐他汀14 d,另选健康SD大鼠作为对照组。以经修改神经功能损伤程度评分(m NSS)来测定神经功能恢复状态;以淀粉样-β阳性前体蛋白(APP)单克隆抗体进行免疫组化实验以测定轴索损伤程度,以Bielshowshy银染法测定神经轴索密度。结果与安慰剂组及对照组比较,辛伐他汀组m NSS评分明显降低,差异有统计学意义(P<0.05);辛伐他汀组APP阳性神经轴索密度明显降低,而银染阳性神经轴索密度明显增高,差异有统计学意义(P<0.05);m NSS评分与神经轴索密度呈明显负相关。结论辛伐他汀能促进颅脑外伤后弥漫性周索损伤中神经轴索损伤的修复与增长。
Objective To observe the effect of simvastatin on the treatment of diffuse axonal injury（ DAI） after traumatic brain injury（ TBI）in rats. Methods Adult healthy Sprague-Dawley（ SD） rats DAI models after brain injury was made by accelerating and decelerating apparatus,which were then divided by two groups. Saline and simvastatin was administrated for 14 days. A modified neurological severity scores（ mNSS） test was performed to evaluate degree of neural functional recovery. Immunohistochemistry studies with amyloid β-precursor protein（ APP） was performed to mark the axonal injury,and Bielshowshys silver dying was processed to evaluate the axon density. Results Symvastatin decreased the mNSS score in the simvastatin group. The density of APP positive neural axons decreased obviously in the simvastatin goup,with which of silver postive axons elevated in it. The mNSS score was negatively correlated with the density of neural axons through Pearson correlation analysis. Conclusion Simvastatin can repair axonal injury and promote axonal regeneration in DAI after TBI.
出处
《中西医结合心脑血管病杂志》
2016年第23期2766-2769,共4页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
