摘要
樟芝（Taiwanofungus camphoratus）发酵液和经95%乙醇浸提后的菌丝体醇提物分别用石油醚和氯仿进行分级提取,得到石油醚和氯仿提取物。对不同提取物进行体外抑制SPCA-1肺癌细胞增殖实验,发现各提取物均可抑制SPCA-1细胞的增殖,其中发酵液石油醚提取物抑制作用最好,IC50为62.5μg/mL,发酵液和菌丝体氯仿提取物的抑制作用较好,IC50分别为120.9和109.5μg/mL,菌丝体石油醚提取物的抑制作用较差,在25~150μg/mL作用浓度下,抑制率低于20%。各提取物对SPCA-1细胞凋亡和生长周期作用的测定结果表明,各提取物在肺癌细胞SPCA-1中通过诱导细胞凋亡及S期周期阻滞来表现其抑制作用。
Taiwanofungus carnphoratus mycelium was grown in shaken （150 r/min）250 mL flasks containing 100 mL potato dextrose broth and 10% liquid spawn inoculum, and incubated for 6 d at 26℃. Mycelium was harvested by filtration, dried at 60 ℃ to constant weight（26.44 g）, and extracted with 200 mL 95% ethanol for 12 h. After repeating the extraction, the combined supernatants were evaporated to dryness under vacuum and the residue obtained （3.66 g） was first extracted three times with 200 mL petroleum ether followed by three times with 200 mL chloroform. In each case, the combined extracts were again evaporated to dryness under vacuum to yield 0.68 g and 0.03 g of extracted material, respectively. Spent culture fluid was first concentrated five-fold by evaporation under vacuum and extracted sequentially three times with equal volumes of petroleum ether and then three times with equal volumes of chloroform. In each case, the extracts were combined and evaporated to dryness under vacuum to yield 0. 17 g and 0.4 g of residue, respectively. Proliferation of SPCA-1 lung cancer cells in vitro was inhibited by all four extracts. ICs0values of 62.5μg/mL, 120.9μg/mL and 109.5μg/mL were recorded for petroleum ether extracts of spent culture fluid, chloroform extracts of spent culture fluid and chloroform extracts of mycelium respectively, whereas less than 20% inhibition was observed with petroleum ether extracts of mycelium at concentrations of 25-- 150 μg/mL. The inhibitory effects were due to induction of apoptosis and arrest of the cell cycle S-phase.
出处
《食用菌学报》
CSCD
北大核心
2016年第3期67-71,共5页
Acta Edulis Fungi
基金
上海市自然科学基金(16ZR1431000)
山东省博士基金(BS2013SW022)资助
关键词
樟芝
发酵液
菌丝体
细胞凋亡
细胞周期
Taiwanofungus camphoratus
spent culture fluid
mycelium
apoptosis, cell cycle
