摘要
建立高效液相测定水产品中15种激素的鉴别及含量测定方法。水产品经甲醇超声提取,0.45μm滤膜过滤,经高效液相色谱分析。试验证明,15种雌激素得到很好的分离,并得到浓度与峰面积呈正比的线性关系,相关系数R^2>0.999;重复性(N=6)相对标准偏差均在5%以内;定量限:雌三醇0.75μg/g、雌二醇0.45μg/g、炔雌醇0.45μg/g、雌酮0.45μg/g、炔诺酮0.60μg/g、己烯雌酚0.45μg/g、炔诺孕酮0.75μg/g、醋酸氯地孕酮0.75μg/g、醋酸甲地孕酮0.75μg/g、醋酸甲羟孕酮0.45μg/g、黄体酮0.45μg/g、苯甲酸雌二醇0.60μg/g、戊酸雌二醇0.75μg/g、已酸羟孕酮0.60μg/g、庚酸炔诺酮0.75μg/g;加标回收率均在80%~120%之间。证明该方法用于检测水产品中的激素具有快速、简便、灵敏、准确且样品处理简单等优点。
Establish a method to determination 15 kinds of hormone in shrimp by HPLC together. Samples were disposed by methanol ultrasonic extraction, including 0.45 μm filter membrane filtration, by high performance liquid chromatography （HPLC）. The experiments showed that 15 kinds of hormone were separated from each other and obtained linear relationship with concentration and peak area. The correlation coefficient R^2〉0.999; Repeatability： RSD〈5%; LOQ： Estriol was 0.75 μg/g; Estradiol was 0.45μg/g; Ethinylestradiol was 0.45 μg/g; Estrone was 0.45 μg/g; Norethindrone was 0.60μg/g; Diethylstilbestrol was 0.45 μg/g; Norgestrel was 0.75 μg/g; Chlormadinone acetate was 0.75 μg/g; Megestrol acetate was 0.75 μg/g; Medroxyprogesterone acetate was 0.45μg/g; Progesterone was 0.45μg/g; Estradiol benzoate was 0.60μg/g; Pentanoic acid estradiol was 0.75μg/g; Hydroxyprogestrone caproate was 0.60 μg/g; Norethisterone enantate was 0.75μg/g; The recoveries for samples ranged from 80%-120%. This method was proved to be rapid, simple, sensitive, accurate and simple sample treatment, etc.
出处
《食品工业》
CAS
北大核心
2017年第1期310-314,共5页
The Food Industry
关键词
水产品
避孕药
方法
测定
高效液相
aquatic products
birth control pills
method
determination
HPLC
