摘要
目的 开发一种简易的分析肿瘤细胞趋电性迁移的微流控芯片技术。方法 微流控肿瘤细胞电趋化芯片基本结构由直微通道和微通道两侧的储液池组成,通过插入储液池内的两支铂丝电极对微通道施加强度可控的直流电场; 微通道内电场分布和强度通过有限元分析软件COMSOL Multiphysics和实验测试进行表征; 以迁移总距离、迁移平均速度、x方向前进迁移指数（x FMI）和y方向前进迁移指数（y FMI）作为量化参数,分析横纹肌肉瘤RD细胞在不同强度直流电场下的趋电性迁移行为。结果 有限元分析结果和实验测量结果显示,所设计的微流控电趋化芯片结构能够在微通道内获得均匀分布且强度可控的直流电场; 细胞趋电性实验显示横纹肌肉瘤RD细胞朝电场阳极迁移; 在188~1320 V/m的直流电场范围内,RD细胞的x FMI和迁移距离随电场强度增大而增加。结论该研究开发的肿瘤细胞趋电性迁移分析微流控芯片技术方法简单易行,有望用于各种肿瘤细胞及正常细胞的电趋化迁移行为及机制分析。
Objective To develop a simple microfluidic chip technology for analyzing the electrotaxis of cancer cells. Methods The basic structure of the proposed microfluidic electrotaxis chip included a straight microchannel and liquid storage pools located on both sides of the microchannel. Two platinum electrodes were inserted into the liquid pools to create a controllable direct current （DC） field in the microchannel. The distribution and strength of the DC field in the microchannel was analyzed by the finite element analysis software COMSOL muhiphysics and experiment tests. Finally, the electrotactic behavior of the rhabdomyosarcoma RD cells in the DC field of different strength was characterized using the accumulated distance, average velocity, x forward migration index （xFMI） and y forward migration index （yFMI） as quantitative parameters. Results The results of element analysis and experiments showed that the structure of the designed microfluidic electrotaxis chip was able to guarantee a uniform and strength-controllable DC field in the microchannel. The experiment of cell electrotaxis showed lhal the RD cells migrated toward the anode of the DC field. Meanwhile, the values of xFMI and accumulated distance for RD cells increased with the enlargement of the DC field, with the strength ranging from 188 to 1320 V/m. Conclusion The microfluidic chip technology developed in this paper for assaying the electrotaxis of cancer ceils is simple and easily implementable, and it can be used for studies of the electrotactic behavior and underlying mechanisms of various cancer cells and normal ceils in the future.
作者
米永华
肖文海
廖娟
陈静
李远
MI Yong-hua XIAO Wen-hai LIAO Juan CHEN Jing LI Yuan(Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China Traditional Chinese Medicine Hospital of Dazu District, Chongqing 402360, China)
出处
《军事医学》
CAS
CSCD
北大核心
2016年第12期946-951,957,共7页
Military Medical Sciences
基金
重庆市自然科学基金面上资助项目(cstc2012jj A10046)
重庆市卫生局中医药科技资助项目(ZY20132132)
重庆市永川区创新能力建设平台资助项目(Ycstc2014bf5001)
重庆市大足区科技计划资助项目(DZKJ
2014ACC1084)
重庆医科大学附属永川医院院级重点研究资助项目(YJZD201302)
