摘要
以供氧策略和进水氨氮浓度为控制因素,应用PCR-DGGE技术并结合系统处理效果研究SBR内活性污泥微生物群落结构的演替情况。结果表明,在一定范围内,进水氨氮浓度越小越有利于COD和氨氮的去除,同时污泥性状主要受供氧时间的影响,运行前40 d,MLSS值均在4 000 mg·L^(-1)以上波动。微生物群落结构受供氧的影响演替剧烈,多样性指数在时间上呈先增后减的变化趋势,曝停比为4 h∶2 h的间歇曝气B方案下菌种最丰富,多样性指数达1.092、1.079。切胶测序结果显示,SBR系统内微生物菌种大部分为未培养菌种,其中酸杆菌属和绿弯菌属占据优势地位,这些菌种在污水生物处理中对污泥性能和系统的运行有重要的影响。
Aeration strategy and ammonia concentration were employed as the control factors to explore the succession of the microbial community structure in SBR activated sludge. We utilized PCR-DGGE technology and the treatment effects of the SBR system in this research. The results indicated that a lower influent ammonia concentration was more beneficial for the removal of COD and ammonia within a certain range. However,the sludge characteristics were affected mainly by the aeration period,with the MLSS value being approximately 4 000 mg·L- 1during the initial 40 days of operation. In addition,the aeration strategy affected the microbial community that experienced a drastic succession,with the change trend of the diversity index over time initially increasing and subsequently decreasing. The microbial species was most abundant in stage B of intermittent aeration,with a4 h ∶ 2 h aeration / non-aeration ratio,and the diversity index reaching 1. 092 and 1. 079. The rubber cutting sequencing showed that the majority of bacteria in the SBR were uncultured bacteria,among which Acidobacteria and Chloroflexi held a dominant position. This played an important role in the sludge performance and the operation of the system in the biological treatment of sewage.
出处
《环境工程学报》
CAS
CSCD
北大核心
2017年第1期359-365,共7页
Chinese Journal of Environmental Engineering
关键词
供氧策略
氨氮浓度
PCR-DGGE
SBR
活性污泥
微生物群落结构
aeration strategy
concentration of ammonia nitrogen
PCR-DGGE
SBR
activated sludge
microbial community structure
