摘要
为提高菌根真菌液体发酵菌丝体生物量,以外生菌根真菌劣味乳菇（Lactarius insulsus）为试验材料,采用液体发酵法,分析接种量、溶解氧及p H值等因子对其发酵培养的影响,并将发酵产物制成液体菌剂,采用灌根法对杨树溃疡病进行田间防治试验。结果表明,接种量5%,发酵过程中p H保持5左右,发酵中后期保持溶氧浓度在28%~35%可有效提高劣味乳菇菌丝体生物量,缩短了发酵周期;田间防治试验表明,劣味乳菇对溃疡病有较好防治效果,防效可达41.1%。该研究可为该菌株作为生防菌株推广应用提供参考依据和理论基础。
To improve the mycelia biomass of mycorrhizal fungi by liquid fermentation,with ECM Lactarius insulsus as experimental material,through the liquid fermentation,the impacts of different concentrations of dissolved oxygen,p H and the inoculation amount on mycelial biomass were analysed,and liquid inoculation against poplar canker was conducted by using the root irrigation method.The results showed that 5% of the inoculum amount,fermentation in the late dissolved oxygen concentration about 28%- 35%,medium maintaining the p H about 5 can effectively improve the mycelial biomass of Li,and shorten the fermentation period. The control effect of Xerocomus chrysenteron against poplar canker reached 41.1% in fields.This study provides the reference and theoretical basis for the industrial liquid fermentation of this strain and its application as a biocontrol strain.
作者
张扬
叶建仁
吴小芹
ZHANG Yang YE Jian-ren WU Xiao-qin(Jiangsu Key Laboratory for Prevention and Management of Invasive Specie, Nanjing Forestry Universi- ty, Nanjing 210037, China College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2016年第6期1161-1166,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家林业公益性行业科研专项(201004061)~~
关键词
劣味乳菇
发酵工艺
溃疡病
防效
Lactarius insulsus
fermentation technology
canker
control effect
