摘要
背景:临床上常将人工虫草多糖作为肾病综合征的辅助药。采用阿霉素建立SD大鼠肾病综合征模型是经典的实验性肾病综合征模型之一,该模型的临床表现及病理变化均类似于人类微小病变肾病。目的:观察人工虫草多糖与脂肪间充质干细胞联合应用对肾病综合征大鼠肾功能及凝血的影响。方法:体外培养脂肪间充质干细胞制备细胞悬液,并对悬液进行PKH26标记。50只SD大鼠随机取10只为正常组(不予任何处理),余40只SD大鼠静脉注射阿霉素建立阿霉素肾病大鼠模型,随机均分为4组,模型组不作处理;脂肪间充质干细胞组尾部静脉注射脂肪间充质干细胞连续3 d;人工虫草多糖组灌胃人工虫草多糖连续12周;联合组连续3 d尾静脉注射干细胞,同时灌胃人工虫草多糖连续12周。实验12周测定各组大鼠24 h尿蛋白定量、血清血脂指标、肌酐、尿素氮及凝血指标变化;光镜下观察双肾组织病理学变化;荧光显微镜观测PKH26标记的脂肪间充质干细胞存活及分布情况,采用RT-PCR技术检测肾组织中Hpa基因表达情况。结果与结论:(1)检测指标:脂肪间充质干细胞组、人工虫草多糖组及联合组的24 h尿蛋白定量及血清总胆固醇、三酰甘油、低密度脂蛋白、肌酐、尿素氮水平均明显低于模型组(P<0.05),而血清总蛋白、白蛋白、高密度脂蛋白水平则明显高于模型组(P<0.05)。联合组各项指标与脂肪间充质干细胞组及人工虫草多糖组比较,差异有显著性意义(P<0.05);(2)血液高凝状态:脂肪间充质干细胞组、人工虫草多糖组大鼠的血液高凝状态较模型组明显缓解,联合组进一步缓解(P<0.05);(3)双肾组织病理变化:联合组的双肾组织病理改善情况明显优于脂肪间充质干细胞组和人工虫草多糖组,脂肪间充质干细胞组和人工虫草多糖组较模型组稍有改善,但不及联合组;(4)荧光显微镜观测PKH26标记的脂肪间充质干�
BACKGROUND：Cordyceps polysaccharide is a commonly used adjuvant drug for clinical treatment of nephrotic syndrome. As a classic model of nephrotic syndrome induced by adriamycin, the Sprague-Dawley rat model of nephrotic syndrome exhibits similar clinical manifestations and pathological changes to minimal-change nephropathy in humans. OBJECTIVE：To observe the effect of adipose-derived mesenchymal stem cel s （ADMSCs） transplantation combined with cordyceps polysaccharide on renal function and hypercoagulable state in rats with nephrotic syndrome. METHODS：ADMSCs suspension was made in vitro and labeled using PKH26. Fifty Sprague-Dawley rats were randomized into normal （no intervention）, model, ADMSCs, cordyceps polysaccharide and combined treatment groups （n=10/group）. Adriamycin administration was performed to make rat models of nephrotic syndrome in the latter three groups. After modeling, model rats were respectively given no treatment, ADMSCs intravenously for 3 days, cordyceps polysaccharide intragastrical y for 12 weeks, or their combined use. Then, 24-hour urinary protein, serum total cholesterol, triglyceride, total protein, albumin, high-density lipoprotein, low-density lipoprotein, creatinine, blood urea nitrogen levels and coagulation changes were detected at 12 weeks. Meanwhile, histopathological changes of renal tissues were observed under light microscope;survival and distribution of PKH26-labeled ADMSCs were observed by fluorescence microscopy;and expression of Hpa gene in renal tissue was detected by RT-PCR. RESULTS AND CONCLUSION：Compared with the model group, the 24-hour urinary protein, serum total cholesterol, triglyceride, low-density lipoprotein, creatinine, and blood urea nitrogen levels were significantly lower, while the serum total protein, albumin and high-density lipoprotein levels were significantly higher in the three treatment groups （P〈0.05）. These indicators showed significant differences between the combined group and ADMSCs and cordyceps polysaccharide gr
作者
郭志伯
张晨洁
马李娜
高大维
宋聚星
李小静
Guo Zhi-bo Zhang Chen-jie Ma Li-na Gao Da-wei Song Ju-xing Li Xiao-jing(Department of Hematology and Nephrology Department of Dialysis Department of Gynecology and Obstetrics Clinical Laboratory Department of Pathology, Baoding Second Central Hospital, Baoding 072750, Hebei Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2016年第50期7549-7556,共8页
Chinese Journal of Tissue Engineering Research
