摘要
目的探讨焦虑/抑郁样行为对小鼠空间学习记忆能力的影响及其与海马区胰岛素样生长因子2(IGF2)水平的关系。方法采用强迫游泳诱导小鼠出现焦虑/抑郁样行为,利用旷场实验和Morris水迷宫实验评价小鼠的焦虑/抑郁样行为和空间学习记忆能力,利用实时荧光定量PCR技术检测海马区IGF2及IGF2受体表达水平,检测糖皮质激素受体(NR3C1)及NR3C1的负性调控因子FK506结合蛋白5(FKBP5)表达水平。结果旷场实验表明强迫游泳导致小鼠出现焦虑/抑郁样行为;Morris水迷宫实验测试表明强迫游泳导致小鼠空间学习记忆能力下降,主要表现为记忆巩固和重现异常。实时荧光定量PCR检测结果表明强迫游泳小鼠海马区IGF2表达水平下降,但NR3C1表达水平升高,均P<0.05。结论强迫游泳导致小鼠出现焦虑/抑郁样行为改变,导致其空间学习记忆能力下降,这一行为学改变与海马区NR3C1升高及IGF2水平下降有关。
Objective To explore the effect of anxiety-like/depression-like behaviors on spatial learning and memory ability and the relationship between this effect and hippocampal insulin-like growth factor 2（IGF2） level. Methods Anxiety-like/depression-like behaviors were induced by forced swimming （FS）. Anxiety-like/ depression-like behaviors and spatial learning and memory ability were evaluated by open-field test and Mor- ris water maze test respectively. The mRNA levels of IGF2, IGF2R and Glucocorticoid receptor （NR3C1） as well as the negative regulatory factors of NR3C 1, FK506 binding protein （FKBP5）in the hippocampus were analyzed using quantitative real-time PCR（Q-RT-PCR） technology. Results Open-field test showed that F S led to anxiety-like/depression-like behavior and Morris water maze test showed that FS impaired the spatial learning and memory ability in mice, which mainly characterized by impairment memory consolidation and re- currence. The detection result of Q-RT-PCR showed that the behavioral alterations were accompanied by the decrease in the mRNA expression level of IGF2 and the increased expression level of NR3C1 in the hippocam-pus. Conclusion FS lead to the anxiety-like/depression-like behaviors and the spatial learning and memory ability impairment in mice. Behavioral change is associated with the increased NR3C 1 level and the decreased IGF2 level.
出处
《兰州大学学报（医学版）》
CAS
2016年第6期8-14,共7页
Journal of Lanzhou University（Medical Sciences）
基金
甘肃省自然科学基金项目(145RJZA073)
国家自然科学基金项目(81570725)
关键词
胰岛素样生长因子2
海马
抑郁
学习记忆
insulin-like growth factor 2
hippocampus
depression
leaming and memory
