摘要
目的:研究过氧化氢人脐静脉内皮细胞(H_2O_2)对白细胞介素(IL)-37表达的影响。方法:用实时荧光定量PCR法和Western blot法检测经系列浓度H_2O_2作用不同时间的人脐静脉内皮细胞中IL-37 mRNA和蛋白水平的变化。结果:实时荧光定量PCR结果显示:不同浓度H_2O_2作用人脐静脉内皮细胞后,IL-37 mRNA水平升高,并呈浓度依赖(P<0.01)。Western blot结果表明,H_2O_2作用人脐静脉内皮细胞20 h后,IL-37蛋白水平较对照组显著升高(P<0.01)。结论:H_2O_2能够上调人脐静脉血管内皮细胞IL-37的表达,为进一步研究IL-37在细胞氧化应激状态下的作用奠定了一定基础。
Objective：To investigate the effects of hydrogen peroxide （ H2 O2 ） on the expression of interleukin-37 （ IL-37 ） in EA. hy926 cells. Methods：The expressions of IL-37 mRNA and protein in different time-points of human umbilical vein endothelial cell treated with different concentration of H2 O2 were detected by qRT-PCR and Western blot. Results：The results of qPCR showed that the levels of IL-37 mRNA in human umbilical vein endothelial cell treated with different concentration of H2 O2 significantly increased, which was the concentration-dependent（P 〈0. 01）. The results of Western blot showed that the level of IL-37 protein in human umbilical vein endothelial cell treated with H2O2 for 20 h was significantly higher than that in control group（P〈0. 01）. Conclusions：The expression of IL-37 in human umbilical vein endothelial cell can be up-regulated by H2 O2 ,which may be helpful to study the role of IL-37 in cellular oxidative stress.
作者
刘倩
周庆
LIU Qian ZHOU Qing(Department of Clinical Laboratory, The Affiliated Xuzhou Center Hospital of Nanjing University of Chinese Medicine,Xuzhou Jiangsu 221009, China)
出处
《蚌埠医学院学报》
CAS
2016年第11期1413-1416,1421,共5页
Journal of Bengbu Medical College
