摘要
目的研究蒲公英中桉叶烷型倍半萜类化学成分。方法采用多种色谱技术从蒲公英提取物中获得单体化合物,MS和NMR法确定化合物结构;以LPS诱导的小鼠腹腔巨噬细胞释放NO为靶点,测试单体化合物的抗炎活性。结果从蒲公英全草中分离并鉴定了8个化合物,分别为plebeiolide A(1),plebeiafuran(2),9β-acetoxy-1β-hydroperoxy-3β,4β-dihydroxygermacra-5,10(14)-diene(3),4-O-acetyl-3-O-(3'-acetoxy-2'-hydroxy-2'-methylbutyryl)-cuauhtemone(4),chlorantholide A(5),chlorantholide C(6),daucucarotol(7)和1α,6β-dihydroxy-cis-eudesm-3-ene-6-O-β-D-glucopyranoside(8)。化合物1、3、4和6具有较强的抑制NO生成作用,具有潜在的抗炎活性,IC50值分别为2.8、3.6、9.5和7.5μmol·L^(-1)。结论化合物1~8为首次从蒲公英中分离获得。
Objective To investigate the chemical compounds of eudesmane-type sesquiterpenes from Taraxacum mongolicum Hand.-Mazz. Methods The constituents were isolated and purified by chromatographic methods from the 95% Et OH extract of the plant, and the structures were identified by MS and NMR spectral analysis. The activity of the compounds isolated were tested the activity by suppressing lipopolysaccharide-induced NO production in mouse macrophages. Results Eight compounds were obtained and elucidated as plebeiolide A（1）, plebeiafuran（2）, 9β-acetoxy-1β-hydroperoxy-3β, 4β-dihydroxygermacra-5, 10（14）-diene（3）, 4-O-acetyl-3-O-（3＇-acetoxy-2＇-hydroxy-2＇-methylbutyryl）-cuauhtemone（4）, chlorantholide A（5）, chlorantholide C（6）, daucucarotol（7）, and 1α, 6β-dihydroxy-cis-eudesm-3-ene-6-O-β-D-glucopyranoside（8）. Compound 1, 3, 4, and 6 exhibited potential anti-inflammation activity by suppressing lipopolysaccharide-induced NO production in mouse macrophages, with IC50 values of 2.8, 3.6, 9.5 and 7.5 μmol·L^（-1）, respectively. Conclusion Compound 1- 8 have been separated from the plant for the first time.
作者
姜醒
赵敏
高晓波
朱景鑫
吕红梅
王家志
苑光军
JIANG Xing ZHAO Min GAO Xiao-bo ZHU Jing-xin LV Hong-mei WANG Jia-zhi YUAN Guang-jun(Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi Heilongjiang 154007)
出处
《中南药学》
CAS
2016年第12期1293-1297,共5页
Central South Pharmacy
基金
黑龙江省自然基金项目(No.H201464)
关键词
蒲公英
桉叶烷型倍半萜
化学成分
Taraxacum mongolicum
eudesmane-type sesquiterpene
chemical component
