摘要
目的：应用基因分型技术鉴定产生抗-M抗体的患者样本 MN血型，协助解决 MN血型相关的临床输血问题。方法在血清学鉴定的基础上，采用聚合酶链反应-序列特异性引物（PCR-SSP）基因定型方法对14例产生抗-M抗体样本的MN血型进行基因分型，并与血清学结果比较；选取 M抗原阴性的血液与之进行交叉配血。结果14例样本基因分型结果与血清学分型结果相符，其中11例为 NN型，2例为 MN型，1例为 MM型。选取 M抗原阴性的供血者血液与该14例患者血液配血均相合，患者输血后无输血反应，24 h内血红蛋白上升。结论 MN血型基因分型技术可作为血清学方法的补充，有利于临床输血相关疑难样本的检测。
Objective To help solve the problem of clinical blood transfusion related to MN blood group,using genotyping technology to identify MN blood group of the patients’blood sample which exist anti-M antibody.Methods A total of 14 blood samples which exist anti-M antibody were detected for MN blood group by polymerase chain reaction-sequence specific primers （PCR-SSP）and serologic technique,respectively,and compared the genotyping results with serotyping results.Se-lected M antigen negative blood for cross matching with 14 cases.Results 14 cases’s genotyping results and serotyping re-sults were comcidental and in them,NN type of 1 1 cases,MN type of 2 cases,MM type of 1 cases.The M antigen negative blood cross matched with 14 cases were compatible.The patients had no blood transfuse reaction after blood transfusion,and the hemoglobin were increase within 24 hour.Conclusion Genotyping technology is helpful for detecting complicated sam-ples related to clinical blood transfusion.It can be use for the compliment of serologic technique.
出处
《现代检验医学杂志》
CAS
2016年第6期130-132,共3页
Journal of Modern Laboratory Medicine
基金
基金项目:广东河源地区MN血型的基因研究与临床输血应用(2013-066)
关键词
MN血型
基因分型
河源地区
临床输血
MN blood group
genotyping
heyuan area
clinical blood transfusion
