摘要
目的探讨程氏蠲痹汤加减方含药血清对体外培养的佐剂性关节炎(adjuvant arthritis,AA)大鼠外周血单核细胞中肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)和白细胞介素-1β(interleukin-1beta,IL-1β)表达的影响。方法将清洁级雄性SD大鼠随机分成6组:胎牛血清组、程氏蠲痹汤加减方(JBT)组、雷公藤多苷片(TGT)组、JBT+sc-13143组、正常大鼠血清组以及阴性对照组。采用皮内注射弗氏完全佐剂和冷水吹风刺激的方法复制风寒湿痹阻型AA模型。检测各组大鼠血清培养基中外周血单核细胞TNF-α和IL-1β的表达水平。结果 JBT组大鼠外周血单核细胞TNF-α和IL-1β表达水平明显降低,与胎牛血清组、正常大鼠血清组以及阴性对照组比较,差异均有统计学意义(P<0.05)。JBT+sc-13143组与JBT组TNF-α、IL-1β表达水平相比,差异也有统计学意义(P<0.05)。结论程氏蠲痹汤加减方能有效下调AA大鼠外周血单核细胞TNF-α和IL-1β水平。
Objective To investigate the effect of modified Cheng＇s Juanbi Decoction-containing serum on the expression of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in peripheral blood mononu- clear cells cultured in vitro in rats with adjuvant arthritis （AA）. Methods Clean male Sprague-Dawley rats were randomly divided into fetal bovine serum group, modified Cheng＇s Juanbi Decoction （JBT） group, tripterygium glycoside tablet （TGT） group, JBT＋sc-1β43 group, normal rat serum group, and negative control group. A model of cold-damp obstruction-type AA was established by intradermal injec- tion of Freund＇s complete adjuvant and stimulation with cold water and wind. The expression of TNF-α and IL-1β in peripheral blood mononuclear cells in rats was measured. Results The JBT group had signifi- cant reductions in the expression of TNF-α and IL-1β in peripheral blood mononuclear cells compared with the fetal bovine serum group, the normal rat group, and the negative control group （P〈0. 05）. There were significant differences in the expression of TNF-α and IL-1β between the JBT＋sc-1β43 group and the JBT group （P〈0. 05）. Conclusion Modified Cheng＇s Juanbi Decoction can effectively downregulate the expression of TNF-α and IL-1β in peripheral blood mononuclear cells in rats with AA.
出处
《安徽中医药大学学报》
2016年第6期83-86,共4页
Journal of Anhui University of Chinese Medicine
基金
国家自然科学基金青年项目(81302911)
高校省级优秀青年人才基金重点项目(2013SQRL040ZD)
安徽中医药大学自然科学研究项目(2013qn015)
