摘要
目的观察芪仙汤对哮喘模型小鼠的作用,探讨芪仙汤促进磷酸酶基因(PTEN)表达的机制。方法健康雌性BALB/c小鼠28只,随机数字表法分为正常对照组、模型对照组、芪仙汤组和地塞米松组,每组7只。以卵清蛋白(OVA)致敏法建立哮喘模型小鼠,造模成功后给予药物处理2周,采用肺组织苏木精-伊红(HE)染色评价小鼠肺部炎症,过碘酸-希夫(PAS)染色检测气道黏液分泌情况,酶联免疫吸附试验(ELISA)检测血清免疫球蛋白Ig E浓度,实时定量聚合酶链反应检测小鼠肺组织中白细胞介素(IL)-5和IL^(-1)3的基因表达变化,Western blotting法检测小鼠肺组织PTEN、SIRT1的表达。结果与正常对照组比较,模型对照组小鼠肺组织呈明显炎症细胞浸润,气道黏液分泌显著增加(P<0.01);与模型对照组比较,芪仙汤组上述指标明显缓解。模型对照组、正常对照组、芪仙汤组和地塞米松组小鼠血清Ig E含量分别为(6.67±2.59),(0.27±0.05),(3.52±1.44),(2.03±1.24)pg·m L^(-1)。模型对照组小鼠肺组织PTEN和SIRT1的表达量显著低于正常对照组,芪仙汤组则显著高于模型对照组(P<0.05)。芪仙汤组IL-5、IL^(-1)3 mRNA水平显著下降(P<0.05)。结论芪仙汤能显著抑制哮喘模型小鼠气道炎症反应,对Ig E、IL-5、IL^(-1)3具有调节作用;上调SIRT1可能是上调PTEN表达的机制之一。
Objective To observe the effect of qixiantang decoction on asthma model mice and to explore its mechanism of phosphatase gene（ PTEN）-up-regulation. Methods A total of 28 healthy female BALB/c mice were divided into 4 groups according to the random number table（ n = 7） ： normal control group,model control group,qixiantang decoction group,and dexamethasone group. The mice were sensitized with ovalbumin（ OVA） for asthma model. Qixiantang decoction group was treated with drug after OVA sensitization. Hematoxylin-eosin（ H-E） staining was applied to observe the pulmonary inflammation in mice,and periodic acid Schiff（ PAS） staining was used to examine airway mucus secretion. ELISA was used to detect the concentration of serum IgE. Real-time quantitative PCR was used to examine IL-13 and IL-5 gene expression changes in lung tissues of mice. Western blotting was used to detect the expression of PTEN and SIRT1 protein in lung tissues. Results The lung tissue inflammatory infiltration and mucus secretion in model control group were higher than normal control group（ P〈0.01）,and that in the qixiantang decoction group. The level of serum IgE in model control group [（ 6. 67 ± 2. 59）pg·mL^-1） ] was significantly higher than normal control group [（ 0. 27 ± 0. 05） pg·mL^-1,P〈0.01],and that in the qixiantang decoction group [（ 3. 52 ± 1. 44） pg·mL^-1,P〈0.05]. The expression of PTEN and SIRT1 in lung tissue of model control group were significantly lower than normal control group,and that of qixiantang decoction group. The expression of IL-5and IL-13 mRNA of qixiantang decoction group was significantly lower（ P〈0.05）. Conclusion Qixiantang decoction could significantly ameliorate inflammation in asthmatic mice by regulate IgE、IL-5、IL-13 expression,and might up-regulate PTEN expression via SIRT1 signal.
出处
《医药导报》
CAS
2017年第1期16-21,共6页
Herald of Medicine
基金
上海市科学技术委员会2012年科技攻关项目(12401900404)
上海市科学委员会引领计划(10411969100)
上海市教育委员会科研创新项目(13ZZ096)
"085"一流学科建设科技创新支撑计划(085ZY1216)
国家自然科学基金资助项目(81402988)
关键词
芪仙汤
哮喘
磷酸酶基因
Qixiantang decoction
Asthma
Phosphatase gene
