摘要
【目的】研究甘蔗应答水分胁迫差异基因表达,进一步探究甘蔗应答水分胁迫的分子机制,为开展甘蔗抗旱综合性遗传改良研究提供科学依据。【方法】选用GT11为材料,分别构建对照、干旱、复水3个处理总RNA混合池,使用cDNA-SCoT差异显示构建GT11伸长期应答水分胁迫的基因表达谱,筛选、分离TDFs进行测序,在NCBI数据库进行BLAST同源性检索,根据同源基因推测基因功能。【结果】通过42条SCoT引物筛选得到180个TDFs,有100个TDFs与NCBI数据库中已录入的基因具有较高的相似性,根据同源基因功能可分为14类:新陈代谢相关基因(11个)、能量代谢相关基因(12个)、运输途径相关基因(5个)、通信及信号转导相关基因(6个)、细胞循环及DNA加工相关基因(3个)、转录调控因子相关基因(3个)、蛋白质合成相关基因(17个)、蛋白质加工相关基因(1个)、结合功能蛋白相关基因(7个)、环境互作相关基因(3个)、转座子、毒性及质粒蛋白相关基因(5个)、细胞成分生物合成相关基因(1个)、防御相关基因(4个)和未知功能蛋白基因(22个)。【结论】甘蔗应答水分胁迫调节途径具有多样性、复杂性、协调性和网络性;应用cDNA-SCoT差异显示筛选获得水分胁迫相关TDFs,可为进一步研究甘蔗应答水分调控途径的分子机理提供重要信息。
【Objective】The present study was conducted to study the expression of differential genes in sugarcane response to water stress and its molecular mechanism in order to provide scientific basis for developing genetic improvement of sugarcane drought resistant integrity.【Method】Sugarcane cultivar GT11 was used as material to design three treatments viz.control,drought and rewatering.The ＋1^（st） leaves of sugarcane for three treatments were used for extracting total RNA to construct their equal quantity mixed solutions,and the genetic expressing profdes of cultivar GT11 at elongation stage under water stress were constructed by using cDNA-SCoT differential display.The transcript derived fragments（TDFs） were screened and isolated to sequencing,followed by the BLAST homology searching in NCBI database to deduce gene function based on homological genes.【Result】The 180 TDFs were screened by using 42 primers of SCoT marker,of which 100 TDFs were of higher similarity with the accessed genes in NCBI database.According to homologous gene function,these 100 TDFs could be classified into 14 types,which included 11 metabolism related genes,12 energy metabolism related genes,5 transport pathway related genes,6 communication and signal transduction related genes,3cell cycle and DNA processing related genes,3 transcript regulated factor related genes,17 protein synthesis related genes,1 protein processing related genes,7 associated-functional protein related genes,3 interaction with the environment related genes,5 transposons,toxicity and plasmid protein related genes,1 cell component biological synthesis related genes,4 defense related genes,and 22 un-functional protein related genes.【Conclusion】 The regulating pathways of sugarcane response to water stress are of multiformity,complexity,coordination and networked;and the obtained water stress-related TDFs by cDNA-SCoT differential display could provide important information to further explore molecular mechanism sugarcane response to water regulation pa
出处
《南方农业学报》
CAS
CSCD
北大核心
2016年第12期1999-2008,共10页
Journal of Southern Agriculture
基金
National Natural Science Foundation of China(31400281)
Guangxi Natural Science Foundation(2014GXNSFAA118128)
Basic Special Fund of Guangxi Academy of Agricultural Sciences(2015JZ11,2015JM24)
