摘要
为研究95份南瓜资源的遗传关系,提高南瓜育种效率,应用150对简单重复序列(SSR)标记及聚类分析方法对95份核心资源进行遗传多样性分析。结果显示,有32对引物能够在不同南瓜种质间扩增出清晰而稳定的多态性标记。利用MEGA 4.0统计软件中UPGMA聚类分析,95份南瓜的平均遗传距离为0.583,最大为0.9412,最小为0;分支总长度SBL=10.3384。在分支距离为0.39时,32对SSR引物可以将95份资源可以分为3个类群。第Ⅰ类群,主要为印度南瓜,第Ⅱ类群为中国南瓜,第Ⅲ类群71号为美洲南瓜。本研究为种质资源的鉴定评价,优良种质的挖掘以及品种选育提供了参考。
In order to study the relationship of 95 accessions of squash germplasms and improve the efficiency of new varieties screening, 95 squash core collections were detected in polymorphism by using 150 pairs ofSSR primers. The research showed that 32 pairs of SSR primers were effective to amplify clear and stablepolymorphic markers among different squash core collections. These squash core collections were clustered byusing Mega version 4.01. Results showed that genetic distance of 95 squash germplasms varied from 0 to0.9412, the average genetic distance was 0.583, the total length of the branch SBL=10.3384. When the branchlength was 0.39, 95 germplasms were divided into three groups by 32 higher polymorphic SSR primers. Thefirst group was Cucurbita maxima, the second group was Cucurbita maschata, and the third group wasCucurbita pepo. This study provided scientific basis for germplasms evaluation, identification of germplasm resources and breeding of new varieties.
出处
《中国农学通报》
2016年第34期135-142,共8页
Chinese Agricultural Science Bulletin
基金
广东省科技计划项目"重要瓜类蔬菜杂种纯度鉴定的SSR分子标记开发及应用"(2015A020209060)
广东省科技计划项目"香芋南瓜新品种选育研究"(2015A020209059)
广东省科技计划项目"华南特色瓜类遗传图谱构建和分子育种核心技术研发"(2013A061402002)
公益性行业(农业)科研专项"南瓜产业技术研究与示范"(20130311)
