摘要
[目的]建立帚石楠组织培养快速繁殖技术。[方法]以帚石楠为研究对象,从不同消毒方式及时间、不同生长调节剂配比、不同暗处理时间等方面研究帚石楠的组培快繁体系。[结果]处理帚石楠幼嫩茎段时,以0.1%升汞处理12 min较为适宜;最适的分化培养基为MS+BA 0.5 mg/L+1.0 mg/L 2,4-D;最适生根培养基为MS+IBA 0.15 mg/L+NAA 0.25 mg/L;接种后将接种苗进行5 d暗处理可明显提高生根率。[结论]建立了帚石楠组织培养快速繁殖技术体系,为其规模化生产提供理论依据。
[ Objective ] The aim was to establish tissue culture and rapid propagation of CaUuna vulgaris. [ Method] This experiment was con- ducted to investigate in vitro mieropropagation of Calluna vulgaris with the aptical buds excised from the mature elite plants. [ Result] The re- suits showed that the optimum disinfecting time was 12 min with 0.1% Mercuric chloride solution;and the optimum medium of following prolif- eration was MS ＋ BA 0.5 mg/L ＋ 2,4 - D 1.0 rag/L; When the MS ＋ IBA0.15 mg/L ＋ NAA 0.25 mg/L medium induced rooting; Culturing in dark for 5 days coluld promoted rooting. [ Conclusion] The study establish tissue culture and rapid propagation of Calluna vulgaris, and pro- vide theoretical basis for the mass production.
出处
《安徽农业科学》
CAS
2016年第34期150-151,176,共3页
Journal of Anhui Agricultural Sciences
基金
大连市科学技术局科技计划项目"大连市食用菌种质资源库建立及栽培加工技术的产业链建设"(2014B11NC076)
关键词
帚石楠
组织培养
暗培养
Calluna vulgaris
Tissue culture
Rapid propagation
