摘要
沙门氏菌(Salmonella)是常见的食源性致病菌,该菌属型别繁多。沙门氏菌血清型的划分对菌株的分类与鉴定有着重要的意义。本文主要介绍了沙门氏菌的O抗原、H抗原和Vi抗原的特性及结构特征,阐述了多种分子鉴定方法在血清分型检测中应用的进展情况,这些方法大多是以PCR为基础建立的,如多重PCR、基因芯片、高分辨率熔解曲线技术等,使用的引物是根据沙门氏菌的O抗原、H抗原和Vi抗原的编码基因设计的。此外,本文还介绍了两种高通量鉴定方法,通用探针沙门氏菌血清分型法和PremiTest沙门氏菌分型法,但是这两种方法的探针设计比较复杂,使用的设备较为特殊,不易推广。环介导等温扩增(LAMP)方法是等温扩增方法之一,虽然简单、快速、灵敏,但是只适合于单一血清组或血清型的检测。
Salmonella is a kind of common foodborne pathogen,with many types of bacteria.The classification of Salmonella serotyping has an important significance for the classification and identification of the strains.This review focused on the characteristics and structural features of the O,H,and Vi antigens.The advancements of various molecular methods for Salmonella serotyping were also reviewed.Most of these methods were based on PCR,such as multiple PCR,gene chip,high resolution melt（HRM） technology,etc.,and the primers were designed according to the genes encoding O,G,and Vi antigen of Salmonella.Moreover,this study also introduced two methods of high flux identification,probe method and PremiTest in Salmonella serotyping,but the probe design was more complex and it should be used in the special device,which made these methods not easy to promote.Loop-mediated isothermal amplification（LAMP） was one of the isothermal amplification methods,which was simple,rapid,and sensitive,but only suitable for a single group or serum detection.
出处
《食品安全质量检测学报》
CAS
2016年第10期3947-3951,共5页
Journal of Food Safety and Quality
基金
浙江省科技计划项目(2015C33042)
国家质检总局科技计划项目(2014IK123)
西北农林科技大学基本科研业务费项目(Z109021426)~~
关键词
沙门氏菌
抗原
血清分型
分子鉴定
Salmonella
antigen
serotyping
molecular identification
