摘要
目的:检测HBV感染慢性乙型肝炎患者血清与肝组织中的IL-18与TNF-α水平并分析其与ALT的相关性,探讨IL-18与TNF-α在该病的表达情况。方法:HBV感染患者60例,分成肝功能异常组(n=30),肝功能正常组(n=30);30例健康体检者作为对照组。使用酶联免疫吸附法(ELISA)测定受试者血清中IL-18、INF-α水平,使用全自动生化分析仪测定ALT水平,采用免疫组织化学法(sp)检测HBV感染患者肝组织中的IL-18、TNF-α表达情况,并探讨HBV感染慢性乙型肝炎患者血清与肝组织中IL-18、TNF-α与ALT的相关性。结果:IL-18、TNF-α在肝功能异常组与肝功能正常组的肝组织中均可见表达,且肝功能异常组IL-18、TNF-α的表达均高于对照组(P<0.05)。肝功能异常组血清IL-18、TNF-α与ALT水平均高于对照组、肝功能正常组(尸<0.05);肝功能正常组血清IL-18、TNF-α与ALT水平均高于对照组(尸<0.05)。肝功能异常组血清中IL-18和TNF-α水平与ALT水平呈正相关(分别为r=0.607,P<0.05;r=0.574,P<0.05)。结论:细胞因子IL-18与TNF-α在慢性乙型肝炎患者肝功能异常时比肝功能正常时,无论是血清水平还是肝脏组织中的表达均要高。提示其参与了肝细胞炎性损伤的过程,可用于临床病情的评估和预后的判断。
Objective： To study the expressions and correlation of IL-18 and TNF-c~ in the hepatic tissues and peripheral blood of the pa- tients with HBV-related chronic liver disease. Methods： Sixty patients with HBV in our hospital from January 2015 to January 2016 were di- vided into abnormal liver function group （ n = 30） and normal liver function group （ n = 30 ）, 30 cases of healthy persons as control group. Compared the positive expression rates of IL-18 and TNF-α between abnormal liver function group and normal liver function group and ana- lyzed the correlation between the expression of IL-18 ,TNF-α and ALT in the abnormal liver function group. Results： Compared with normal liver function group, abnormal liver function group IL-18 and TNF-αexpressions were higher in the hepatic tissues （P 〈 0.05 ）. The levels of serum IL-18, TNF-and ALT in abnormal liver function group were higher than those in control group and normal liver function group （P 〈 0.05 ）. The levels of serum IL-18, TNF-α and ALT were higher than those in control group （ P 〉 0. 05 ）. Pearman correlation analysis showed that in the abnormal liver function group, the expression of IL-18 positively correlated with ALT （r = 0. 607, P 〈 0.05 ） ;the expression of TNF-α positively correlated with ALT（ r = 0. 574 ,P 〈 0.05 ）. Conclusion： The IL-18 and TNF-α were involved in the occurrence of the patients with HBV-related chronic liver disease , and reduce the expression of IL-18, TNF-α, repair the hepatic tissue is a new way of chronic hepatitis B treatment.
出处
《中西医结合肝病杂志》
CAS
2016年第6期341-343,350,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
