摘要
目的 食管癌是一种常见的上消化道恶性肿瘤,放射治疗是食管癌的主要治疗方法之一。本研究旨在探讨食管鳞癌组织中AGGF1和HIF-1α及VEGF的表达,及其与食管鳞癌临床病理特征、放疗疗效及预后的相关性。方法 采用免疫组化SP法,检测新疆医科大学附属肿瘤医院胸腹放疗科2011-01-01-2015-01-01收治的79例食管鳞癌和20例正常食管组织中AGGF1、HIF-1α和VEGF的表达,结合临床病理特征和随访资料进行相关分析。结果 79例食管鳞癌中AGGF1（χ^2=18.975,P〈0.001）、HIF-1α（χ^2=26.884,P〈0.001）和VEGF（χ^2=22.702,P〈0.001）的表达显著高于20例正常食管组织中的表达。食管鳞癌中AGGF1（χ^2=8.134,P=0.004）和VEGF（χ^2=5.175,P=0.023）的表达与淋巴结转移密切相关;HIF-1α的表达与临床分期（χ^2=5.882,P=0.015）、淋巴结转移（χ^2=7.712,P=0.005）和浸润深度（χ^2=5.538,P=0.019）密切相关。食管鳞癌中VEGF和HIF-1α的表达呈正相关,r=0.333,P=0.003;AGGF1和VEGF的表达呈正相关,r=0.790,P〈0.001。放疗近期疗效与HIF-1α的表达呈负相关,r=-0.246,P=0.029。AGGF1（P=0.170）和VEGF（P=0.229）阳性表达组OS短于阴性组;HIF-1α阳性表达组OS显著短于阴性组,P=0.013。Cox多因素回归分析显示,HIF-1α阳性表达（P=0.002）、浸润深度（P=0.005）和临床分期（P〈0.001）是食管鳞癌患者生存预后的独立因素。结论 AGGF1,HIF-1α及VEGF在食管鳞癌组织中高表达,并与食管鳞癌临床病理特征密切相关,三者高表达可能对食管鳞癌的放疗疗效和生存预后有影响,可为提高食管鳞癌的放疗效果和改善生存预后提供新的思路和方法。
OBJECTIVE Esophageal cancer is a common upper gastrointestinal malignant tumor and radiotherapy is one of the main treatment of esophageal cancer. Our study is to investigate the expressions of AGGF1, HIF-1α and VEGF in esophageal squamous cell carcinoma（ESCC） and their relationships with clinicopathological features, curative effect of radiotherapy and prognosis of ESCC. METHODS The expressions of AGGF1, HIF-1α and VEGF in 79 cases of ESCC and 20 cases of normal esophageal tissue were examined using SP immunohistochemical staining and were analyzed according to the clinicopathological features and follow-up data. RESULTS The expressions of AGGF1 （χ^2=18.975, P〈 0. 001）, HIF-1α（2-2 =26. 884,P〈0. 001） and VEGF（χ^2=22. 702,P〈0. 001） in 79 cases of ESCC were significantly higher than those in 20 cases of normal esophageal tissue. The expressions of AGGF1 （χ^2=8.134,P=0. 004） and VEGF （χ^2= 5. 175,P= 0. 023） in ESCC were significantly related to the lymph node metastasis;The expression of H IF-1α was significantly related to the clinical stage（χ^2= 5. 882 ,P= 0. 015）, lymph node metastasis（χ^2=7. 712, P= 0. 005） and the depth of invasion（χ^2= 5.538, P=0. 019）. The expression of VEGF in ESCC was significantly positively related to the ex- pression of H IF-1α（r= 0. 333, P=0. 003） ;The expression of AGGF1 was significantly positively related to the expression of VEGF（r=0. 790,P〈0. 001）. The radiotherapy curative effect was significantly negatively related to the expression of HIF-1α（r=-0.246,P=0. 029）. The OS of the positive teams of AGGFI（P=0. 170） and VEGF（P〈0. 229） was shorter than that of the negative teams; The OS of the positive team of HIF-1α was shorter than that of the negative team significantly（P= 0. 013）. Cox-proportional multivariate analysis showed that positive expression of HIF-1α（P〈0. 002）, depth of invasion（P= 0. 005）and clinical stage（P〈0. 001）were significant prognostic factor in overall survival. CON
出处
《中华肿瘤防治杂志》
CAS
北大核心
2016年第17期1159-1164,共6页
Chinese Journal of Cancer Prevention and Treatment
