摘要
油茶是世界四大木本油料之一,在中国广泛种植。中国油茶种质资源极为丰富,不同地区油茶存在遗传上的显著多样性。为了掌握油茶资源的遗传多样性,本研究运用ISSR分子标记技术,对来自海南、广西、江西共55份油茶资源进行多态性和聚类分析,分析各品种资源间的遗传关系。研究结果表明:(1)从100条ISSR引物中筛选出13条引物进行PCR扩增,共扩增出110条DNA条带,多态性条带107条,多态率达97.3%。(2)当相似系数为0.612时,广西(5株),江西(4株)油茶品种与海南品种(46株)具有遗传的亲缘关系,但是有较大的遗传差异性。(3)海南品种油茶在亲缘系数为0.676时可分为7个居群,但主要是澄迈、省农科院、琼海聚类的居群占优,说明海南种虽然亲缘性较高,但仍有地区的差异性。
Camellia oleifera is one of the four woody oil plants in the world, which was planted widely in our country. The resource Camellia oleifera in our country is very rich, and it displays remarkable diversity on genetically in different area. In order to study genetic diversity of Camellia oleifera in Hainan province, ISSR molecular marker was used to make polymorphism and cluster analysis to 55 lines of Camellia oleifera from Hainan, Guangxi and Jiangxi province, and analyzing the genetic relationships between variety resources. The results showed that:(1) After amplified 13 primers from 100 ISSR primer pools, total 110 DNA bands were amplified,of which 107 were polymorphic, and the polymorphic rate was 97.3 %.(2) There were close genetic relationships between Camellia oleifera from Guangxi(5 lines), Jiangxi(4 lines) and Hainan(46 lines) when the similarity coefficient was 0.612, but the genetic diversity was significant.(3) The Camellia oleifera from Hainan Province could be divided into 7 groups when the similarity coefficient was 0.676, and the dominant groups were from Chengmai,Hainan Academy of Agriculture, and Qionghai. The results revealed that there were not only high level genetic relationships among Camellia oleifera in Hainan Province, but also evident region differences.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期517-523,共7页
Molecular Plant Breeding
基金
海南省重大科技专项(ZDZX2013023)子课题
中央财政支持中西部高校提升综合实力专项子课题
中西部提升计划研究生资源考察项目海南山柚种质资源考察与研究共同资助
关键词
油茶
ISSR分子标记
遗传多样性
Camellia oleifera
ISSR molecular marker
Genetic diversity
