摘要
利用基因序列对芭蕉属植物的组间及种间的系统发育进行研究可弥补形态特征和生理特性的不足,从而为研究芭蕉属植物的进化与分类提供新的证据。本研究采用PCR产物直接测序的方法对66份芭蕉属野生种质资源r DNA ITS序列进行系统发育研究,结果表明:基于不同的基本染色体数,供试材料能明显地划分为两大类,其中Eumusa组和Rhodochlamys组材料聚到一组,Callimusa组、Australimusa组和Ingensmusa组的材料聚到一组;但是组间材料交叉聚类,具有紧密联系,无法区分。建议将芭蕉属下分类合并为Eumusa-Rhodochlamys组和Callimusa-Australimusa组,取消Ingensmusa组的分类。由于Rhodochlamys与M.acuminata和M.balbisiana的紧密联系,Rhodochlamys组材料可考虑作为香蕉遗传改良的材料,需进一步确定为栽培蕉提供独特S基因组的M.schizocarpa与M.balbisiana的联系以及M.balbisiana的种内分类。
Using DNA sequence data to analyze infrageneric and interspecific phylogeny can provide new insight into the evolution and classification of genus Musa which typically classified using morphological and physiological characteristics. In this study, we sequenced the ribosomal internal transcribed spacer region of 66 accessions of Musa species to analyze the phylogenetic diversity. The results showed that due to the variation in chromosomal numbers, Sect. Eumusa and Sect. Rhodochlamys, as well as Sect. Callimusa, Sect. Australimusa and Sect. Ingensmusa could be relatively close clustered, although the 2 sections could not be separated from each other. We suggested that these two groups of sections should be reclassified together, the classification of Sect.Ingensmusa should be removed. Some species of Sect. Rhodochlamys clustered with the M. acuminata complex and M. balbisiana suggesting they may be sources of useful genes for the improvement of the cultivated bananas.We plan to identify the relationship between M. balbisiana and M. sanguinea which is considered to have the unique S genome and also the intraspecific classification of M. balbisiana.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期442-449,共8页
Molecular Plant Breeding
基金
农业部热带作物种质资源保护项目(15RZZY-46)
海南省自然科学基金项目(314083)
海南省高等学校科学研究项目(Hjkj2013-45)共同资助
