摘要
通过结合废水处理与能源微藻培养,既可以实现废水的无害化处理,也可以为微藻的培养提供营养组分和大量水源.以莱茵衣藻(Chlammydomonas reinhardtii)为实验材料,以酒糟废水为培养基,构建室内微型生态系统,考察了不同处理方法下的酒糟废水接种莱茵衣藻后的总氮(TN)、总磷(TP)、化学需氧量(COD)的去除情况以及微藻蛋白提取情况.结果表明:酒糟废水稀释液初始TN质量浓度位于9.39 ~30.86 mg·L-1时,废水总氮去除率在70%~80%之间;初始TP质量浓度位于3.45~10.93 mg·L-1时,总磷去除率较高,最高达到86.59%;不同稀释倍数的酒糟废水COD去除率均高于50%.而稀释80倍、50倍以及 50倍(氮磷质量浓度比为108∶7)的藻可溶性蛋白增长均比较明显,最大值可达到11.36 mg·L-1,同时也说明通过外加氮、磷调节氮磷的初始质量浓度比至108∶7,可大大提高其产量.总体上,在稀释倍数为50倍(TN、TP初始质量浓度分别为18.23、6.99 mg·L-1)时,莱茵衣藻生长良好,废水中TN、TP、COD去除效果均比较明显,对水质的净化效果最佳,同时可获得较高质量浓度的藻可溶性蛋白,这为酒糟废水与微藻耦合规模化培养提供了一种新的思路.
It could realize innocent treatment of wastewater and provide culture of microalgae with nutrition and water resource by combining wastewater treatment with microalgae culture.In this research,indoor micro ecological system was set by using Chlammydomonas reinhardtii as experimental material and vinasse wastewater as culture medium to observe the removal rate of total nitrogen (TN),total phosphorus (TP) and chemical oxygen demand (COD) in vinasse wastewater,and the effect of microalgae protein extraction as well.The result showed that the final removal rate of TN reached up to 70% to 80% when the initial concentration of TN in vinasse wastewater was between 9.39 mg·L-1 and 30.86 mg·L-1,it had a good removal rate even to 86.59% of TP when the initial content of TP was between 3.45 mg·L-1and 10.93 mg·L-1.C.reinhardtii could remove more than 50% COD of vinasse wastewater which had different dilution ratio.Meantime,the content of soluble protein increased obviously under the conditions of diluted 80×,50×,and 50× (the rate of nitrogen and phosphorus being 108∶7).In general,at the condition of diluted 50× (TN,TP concentration being 18.23 mg·L-1,6.99 mg·L-1,respectively),C.reinhardtii grows well,and TN,TP,COD removal efficiency are more obvious,the water quality purification effect is the best,and it also can obtain a higher content of soluble protein,which provides a new way of thinking for co-cultivation between vinasse wastewater and microalgae.
作者
余江
王萍
冉宗信
赵亮
杨春
YU Jiang WANG Ping RAN Zhongxing ZHAO Liang YANG Chun(College of Architecture and Environment, Sichuan University, Chengdu 610065, Sichuan Institute of New Energy and Low Carbon Technology, Sichuan University, Chengdu 610065, Sichuan Institute of Solid Waste and Hazardous Chemicals Management, Chengdu Academy of Environmental Science, Chengdu 610072, Sichuan College of Chemistry and Materials, Sichuan Normal University, Chengdu 610066, Sichuan)
出处
《四川师范大学学报(自然科学版)》
CAS
北大核心
2016年第6期900-904,共5页
Journal of Sichuan Normal University(Natural Science)
基金
国家自然科学基金(31100374)
成都市科技局项目(2015-HM01-00013-SF)
关键词
酒糟废水
莱茵衣藻
总氮
总磷
COD
可溶性蛋白
vinasse wastewater
Chlammydomonas reinhardtii
total nitrogen
total phosphorus
COD
soluble protein