摘要
[目的]评价别直参对MCF-7细胞体外增殖的影响,并检测其对凋亡相关蛋白bax/bcl-2表达的影响。[方法]采用MTT比色法检测对MCF-7细胞增殖的作用;流式细胞术检测对MCF-7细胞凋亡的影响;免疫组化法检测bax/bcl-2的表达。[结果]别直参高剂量组作用MCF-7细胞24h后,MTT检测增殖率为89.83%,提示有抑制MCF-7细胞体外增殖的作用(P<0.05);流式细胞术检测高剂量组细胞凋亡率明显升高(P<0.05);免疫组化法检测高剂量组能上调MCF-7细胞bax的表达,并下调bcl-2的表达。[结论]别直参高剂量组具有一定的诱导MCF-7细胞凋亡效应作用,其机制可能通过上调bax的表达和下调bcl-2的表达。
[Purpose] To investigate the impact of serum containing Korean Ginseng on MCF-7 cell proliferation and the expression of bax/bal-2 proteins. [Methods] By using the MTT method to detect cell proliferation and flow cytometry to examine cell apoptosis,while the bax/bcl-2 proteins were detected by IHC. [Results] The high dose of Korean ginseng could make an anti-proliferative effect on MCF-7 cell with the proliferation rate of 89.83% by MTT method as well as high apoptosis rate by flow cytometry（P0.05）. The IHC revealed high dose of Korean ginseng increase the expression of bax protein and decrease the expression of bcl-2 protein. [Conclusion] The high dose of Korean ginseng induced apoptosis in MCF-7 cell by increasing the expression of bax protein and decreasing the expression of bcl-2 protein.
出处
《中国肿瘤》
CAS
2016年第12期1004-1008,共5页
China Cancer
基金
浙江省中医药科技计划项目(2012ZB041)
