摘要
采用序批式污泥反应器(SBR),接种好氧颗粒污泥(AGS),以人工模拟低COD/N废水为进水水质,在pH 7.5~8.5,温度为30℃,DO浓度约为0.8 mg·L-1的条件下,通过逐步提高进水氨氮浓度的策略启动部分半硝化工艺.结果表明,通过逐步提高进水氨氮浓度方法,可在60 d内初步形成部分半硝化工艺.部分半硝化工艺运行期间,系统对NO_2^--N的积累率基本维系在80%以上,对TN的去除率平均为64.54%,出水亚硝态氮和氨氮(NO_2^--N/NH_4^+-N)比值平均达到1.16,符合厌氧氨氧化(ANAMMOX)反应进水要求.同时,基于高通量测序技术分析了部分半硝化工艺启动后微生物种群结构组成,其主要优势菌属有:Candidate-division-TM7-norank(相对丰度68.63%)、Saprospiraceae-uncultured(8.26%)、Thauera(4.63%)、Denitratisoma(3.16%)、Anaerolineaceae-uncultured(1.63%)和Anaerovorax(1.39%).部分半硝化AGS-SBR系统中与脱氮功能相关的主要菌属为:Nitrosomonas、Thauera、Denitratisoma和Bacillus,该系统中存在自养脱氮、反硝化和厌氧氨氧化的多种脱氮途径.
The mature aerobic granular sludge（ AGS） was inoculated in an sequencing batch reactor（ SBR） to treat the simulation wastewater with low carbon nitrogen ratio（ COD / N）. The start-up characteristics of partial nitritation（ PN） based on gradually increasing influent ammonia concentration strategy were investigated. The reactor was operated at dissolved oxygen（ DO） of 0. 8mg·L^（-1）,pH 7. 5-8. 5 and 30℃. The PN was realized in the AGS-SBR within 60 days. From day 61 and onwards,the nitrite accumulation efficiency of 80% was achieved throughout the experiment. Meanwhile,the total nitrogen average removal rate was maintained at a relatively high level of 64. 54%,and the effluent NO2^--N / NH4^＋-N ratio reached 1. 16,which was a suitable mixture to feed subsequent anammox. Finally,we also investigated the bacterial abundances in AGS-SBR in the PN period（ PN-AGS-SBR）through Illumina 16 S rRNA gene Mi Seq sequencing. The dominant microbial communities at genus level were subjected to sequence analysis. The results revealed that the relative abundance of Candidate-division-TM7-norank was 68. 63%,Saprospiraceae-uncultured was 8. 26%,Thauera was 4. 63%,Denitratisoma was 3. 16%,Anaerolineaceae-uncultured was 1. 63% and Anaerovorax was 1. 39%,respectively. Nitrosomonas,Thauera,Denitratisoma and Bacillu were considered as the main organisms responsible for nitrogen removal. Meanwhile,various denitrification pathways,such as autotrophic denitrification,the denitrification and anaerobic ammonia oxidation of nitrogen,coexisted in PN-AGS-SBR system.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2016年第12期4741-4749,共9页
Environmental Science
基金
成都市科技惠民技术研发项目(2015-HM01-00477-SF)
成都信息工程大学中青年学术带头人科研基金项目(J201614)
关键词
序批式反应器
好氧颗粒污泥
低COD/N
部分半硝化
高通量测序
sequencing batch reactor
aerobic granular sludge
low COD/N
partial nitritation
high-throughput sequencing
