摘要
目的研究蛤蚧治疗非酒精性脂肪肝(NAFLD)模型小鼠的临床效果。方法选取95只清洁剂雄性昆明小鼠建立NAFLD研究模型,抽签随机分为正常组和模型组,正常组25只给予普通饲料喂养,模型组70只给予高脂饲料喂养,持续喂养6周建立NAFLD模型。模型建立后将模型组中60只抽签随机分为对照组和观察组各30只,两组均给予等渗盐水灌注治疗,观察组加用蛤蚧进行喂养,观察两组血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性以及血脂总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)变化。结果实验小鼠模型成功建立,血脂指标与组织切片与对照组比较,差异具有统计学意义。治疗后观察组ALT(33.54±4.23)IU/L、AST(205.73±20.84)IU/L较对照组(38.75±5.81)IU/L、(221.68±21.25)IU/L明显较低,具有统计学意义(P<0.05)。血脂指标TC(2.82±0.26)mmol/L、TG(1.87±0.36)mmol/L、LDL-C(0.34±0.08)mmol/L较对照组(3.23±0.32)mmol/L、(2.24±0.38)mmol/L、(0.48±0.11)mmol/L明显较低,HDL-C(1.06±0.35)mmol/L较对照组(0.73±0.42)mmol/L明显较高,均具有统计学意义(P<0.05)。结论蛤蚧能有效降低AST、ALT活性。
Objective To research the clinical effect of tokay on treating nonalcoholic fatty liver disease （ NAFLD） of model mice. Methods 95 male cleaner kunming mice were selected to establish the study model of NAFLD, and were randomly divided into normal group and model group. 25 mice of normal group were given normal feed and 70 mice of model group were given a high-fat feed, then they were continuously fed for 6 weeks to build NAFLD model. After the model was established, 60 draw in the model group were randomly divided into control group （ 30 cases） and observation group （ 30 cases） . The two groups were given isotonic saline infusion treatment, and observation group were added with tokay feeding. Serum alanine aminotransferase （ALT）, aspartate aminotransferase （ AST） activity and lipid acyl of total cholesterol （ TC） , three glycerin （ TG） , high-density lipoprotein cholesterol （ HDL-C） , low den-sity lipoprotein cholesterol （ LDL-C） of the two groups were observed. Results The experimental mice model was successfully estab-lished. Compared with control group, lipid indexes and tissue biopsies of observation group had statistically significant difference. ALT and AST of observation group after treatment were significantly lower than the control group and the difference was statistically signifi-cant （ P〈0.05） . Blood lipid index of TC, TG and LDL -C were significantly lower than the control group, HDL-C was significantly higher than the control group, and the difference was statistically significant （ P〈0.05） . Conclusion Tokay can effectively reduce the activity of AST and ALT.
出处
《东南国防医药》
2016年第5期465-467,共3页
Military Medical Journal of Southeast China
基金
河北省中医药管理局计划项目(2015290)
