摘要
胚胎干细胞(ESC)在发育过程中分化为内胚层、中胚层和外胚层3个胚层.其中内胚层进一步向终末细胞的分化,是形成整个消化道和呼吸道,以及肝脏、胰腺等器官的基础.ESC形成内胚层主要经历以下几个分化阶段:外胚叶的分化、原条的形成、内胚层与中胚层的分离以及定型内胚层的形成.本文主要从信号通路、转录因子以及表观遗传调控等几个方面综述胚胎干细胞向内胚层分化的分子机制,并重点介绍其组学研究进展,以期为该领域研究者提供重要参考信息.
The embryonic stem cells （ESC） can be differentiated into three germ layers, known as endoderm, mesoderm and ectoderm. Endoderm, which generates terminally differentiated cells, lays the foundation for generating the respiratory and digestive systems as well as the functional organs such as liver and pancreas. The differentiation of ESC into endoderm includes four main stages： the differentiation into epiblast, the formation of primitive streak, the separation of endoderm and mesoderm, the eventual formation of definitive endoderm （DE）. The molecular mechanisms of endoderm-lineage differentiation of ESC are summarized in this review. We mainly focus on the key signaling pathways, transcription factors, epigenetic regulation mechanisms in the four processes. The progress of the multi-omic studies of these mechanisms are highlighted. This review may provide important referenced information for the researchers in this field.
出处
《中国科学：生命科学》
CSCD
北大核心
2016年第12期1345-1353,共9页
Scientia Sinica(Vitae)
基金
国家自然科学基金(批准号:31671376)
北京市科技新星计划(批准号:Z161100004916148)
国家国际科技合作专项(批准号:2014DFB30020,2014DFB30010)
国家重大科学研究计划(批准号:2015CB910700,2014CBA02001)
蛋白质组学国家重点实验室开放课题(批准号:SKLP-0201404)资助
关键词
胚胎干细胞
内胚层
信号通路
表观遗传
组学
embryonic stem cell, endoderm, signaling pathway, epigenetic, omics
