摘要
目的探讨S14G-Humanin(HNG)对β-淀粉样蛋白(Aβ)致海马CA1区突触长时程增强(LTP)抑制的影响。方法制备小鼠海马脑片(n=20),分为4组:正常对照组、400 n M Aβ25-35组、400 n M Aβ25-35+200 n M HNG组、400 n M Aβ25-35+400 n M HNG组。应用多通道细胞外记录系统(MED系统)记录各组海马脑片兴奋性突触后电位(EPSP)的斜率,并与基础对照的EPSP斜率相比,计算相应的百分比作为LTP数值,观察可溶性Aβ25-35对海马CA1区LTP的抑制作用以及不同浓度HNG对Aβ25-35致LTP抑制效应的影响。结果 400 n M Aβ25-35组LTP值较正常对照组显著减少[(102.5±2.0)%vs(148.1±8.0)%,P<0.01],表明其可显著抑制海马CA1区LTP的产生;400 n M Aβ25-35+200 n M HNG组较400 n M Aβ25-35组LTP值显著增加[(121.3±2.9)%vs(102.5±2.0)%,P<0.01],表明其可部分减少400 n M Aβ25-35对海马CA1区LTP的抑制效应;而400 n M Aβ25-35+400 n M HNG组较400 n M Aβ25-35组LTP显著增加[(143.0±4.3)%vs(102.5±2.0)%,P<0.01],并与正常对照组相比无统计学差异[(143.0±4.3)%vs(148.1±8.0)%,P>0.05],表明其可以完全逆转400 n M Aβ25-35对海马CA1区LTP的抑制效应。结论 HNG能够逆转可溶性Aβ25-35对海马CA1区LTP的抑制作用,提示HNG对Aβ所致的突触可塑性损害具有神经保护作用。
Objective To investigate the influence of S14G-Humanin （HNG）on β-amyloid protein （Aβ）-induced inhibi- ting action of synaptic long-term potentiation （LTP） in hippocampal CA1 region of mice. Methods Twenty mice hipp- ocampal slices were made and divided into normal control group,400 nM Aβ25_35 group,400 nM Aβ25-35 ± 200 nM HNG group ,400 nM Aβ25-35 ± 400 nM HNG group. By multi-channel extracellular recording system （MED system ） , the slope of the excitatory postsynaptic potentials （EPSPs） was measured, and its value was represented by the percentage of measured EPSP slop to the EPSP slope of base control, and the inhibiting effect of soluble Aβ25-35 on synaptic LTP of hippocampal CA1 region and the influence of different concentrations of HNG on inhibiting action of Aβ25-35-induced synaptic LTP in hippocampal CA1 region were observed. Results Compared with normal control group,the LTP of mice hippocampal CA1 region in 400 nM Aβ25-35 group decreased significantly [ （ 102.5 ± 2.0） % vs （ 148. 1 ± 8.0） %, P 〈 0.01 ] , namely, 400 nM Aβ25-35 can significantly inhibit the generation of LTP in hippocampal CA1 region. The LTP value in 400 nM Aβ25-35 ± 200 nM HNG group increased significantly compared with 400 nM Aβ25_35 group [ （ 121.3 ± 2.9） % vs（ 102.5± 2.0 ） % , P 〈 0.01 ] , namely ,200 nM HNG can partially decrease the inhibition effect of 400 nM Aβ25-35 on LTP in hipp- ocampal CA1 region. The LTP value in 400 nM Aβ25.35 ± 400 nM HNG group increased significantly compared with 400 nM Aβ25-35 group [ （ 143.0 ± 4.3 ） % vs （ 102.5 ± 2.0） %, P 〈 0.01 ] and similar with the level of normal control group [ （ （ 143.0 ± 4.3 ） % vs （ 148.1 ± 8.0 ） %, P 〉 0.05 ） ], namely ,400 nM HNG can completely reversed the inhibition effect of 400 nM Aβ25-35 on LTP in hippocampal CA1 region. Conclusions HNG eould reverse soluble Aβ25-35-induced inhibition effect on LTP in hippocampal CA1 region, and this suggests that HNG has neuroprotective effee
出处
《中国临床研究》
CAS
2016年第12期1593-1596,共4页
Chinese Journal of Clinical Research
基金
陕西省自然科学基础研究计划项目(S2014JC9313)
