摘要
目的观察Claudin-1在乳腺癌组织中的表达与临床病理特征的关系，探讨Claudin－1的胞外区Cldn-1 50～80片段在乳腺癌转移中的作用机制。方法（1）应用免疫组织化学技术检测108例乳腺癌标本中Claudin-1的表达情况，并分析其与乳腺癌淋巴结转移、TNM分期和分子分型间的关系；（2）在乳腺癌细胞株中掺入Cldn-1 50～80片段，应用Western blot技术检测紧密连接蛋白Claudiml、Claudin-2的表达。结果（1）以是否淋巴结转移、临床分期、分子分型等病理特征为依据分组中，Claudin-1的表达强度在淋巴结转移组（χ^2=6．279，P=0．012）、TNM分期Ⅲ、Ⅳ期（χ^2=7．419，P=0．024）及分子分型为Her-2过表达型（χ^2=10．830，P=0．013）组中显著降低，差异有统计学意义；（2）Cldn-1 50～80片段的掺入可导致乳腺癌细胞中紧密连接蛋白Claudin-1及Claudin-2的表达下降，在转移性高的乳腺癌细胞株MDA-MB-231中，这种抑制作用更为显著。结论紧密连接蛋白Claudin-1的表达降低与乳腺癌的进展和转移密切相关，而Cldn-1 50～80片段通过调控紧密连接蛋白的表达在乳腺癌细胞侵袭、转移过程中发挥重要作用。
Objective To investigate the expressions of tight junction protein claudin-1 in breast cancer tissue and the regulation with the domaination of extracellular loop Cldn-1 50-80. Methods The expressions of Claudin-1 in the breast cancer samples （n = 108） were detected by immunohistoehemistry technology. The expressions of Claudin-1 and Claudin-2 with the inception of Cldn-1 50-80 were detected by the technology of Western Blot. Results The expression level of Claudin-1 were associated with lymph node metastases,clinical stage and the molecular typing of breast cancer（P〈0.05）. Besides, the inception of Cldn-1 50-80 lead to a lower expression of Claudin-1 and Claudin-2（P〈0.05）. Conclusion Claudin-1 shows a decreasing trend in development and metastases of breast cancer,and Cldn-1 50-80 domain of claudin-1 plays a key role in the regulation of TJ function in breast cancer.
出处
《重庆医学》
CAS
北大核心
2016年第34期4772-4774,共3页
Chongqing medicine
基金
重庆市自然科学基金面上项目(cstc2012jjA10078)
