摘要
[目的]建立同时测定蜂蜜中磺胺类和氯霉素类的超高效液相色谱串联质谱（UPLC-MS）的新方法。[方法]试验以0.2%的甲酸乙腈作为提取剂,通过一种新型的小柱（OasisPRi ME HLB柱）净化处理,流动相定容过膜后上机待测,可以同时提取出蜂蜜中的磺胺类和氯霉素类。[结果]氯霉素在0.1-10.0 ng/m L范围内有良好的线性关系,相关系数大于0.991;磺胺类在0.5-50.0 ng/m L范围内线性关系良好。氯霉素样品最低检出限为0.1μg/kg,磺胺类检出限为0.5μg/kg,回收率在82.50%-101.20%,相对标准偏差为3.2%-4.0%。[结论]该方法快速、准确、灵敏度高,适用于蜂蜜中氯霉素类和磺胺类的检测。
[Objective] To establish a new method UPLC-MS for the determination of sulfanilamide grous and chloramphenicols in honey.[Method]With 0. 2% formic acid acetonitrile as the extraction agent,through purification treatment by a new column（ Oasis PRi ME HLB）,sulfanilamide grous and chloramphenicols can be extracted simultaneously. [Result]Chloramphenicol had a good linear relationship in the range of 0. 1- 10. 0 ng / m L,and the correlation coefficient was greater than 0. 991; sulfanilamide grous had a good linear relationship in the range of 0. 5- 50. 0 ng / m L. The lowest detection limit of chloramphenicol was 0. 1 g / kg,detection limit of sulfanilamide grous was0. 5 μg / kg,recovery was 82. 5%- 101. 2%,relative standard deviation was 3. 2%- 4. 0%. [Conclusion] The method is rapid,accurate and sensitive,and it is suitable for the detection of chloramphenicol and sulfanilamide grous.
出处
《安徽农业科学》
CAS
2016年第31期34-36,39,共4页
Journal of Anhui Agricultural Sciences
