摘要
目的:明确引起栀子褐斑病病原菌种类,为病害防治提供依据。方法:采集江西省不同地区栀子褐斑病病叶标本,采用组织块法对病原菌进行分离纯化,按照柯赫氏法则,用致病性试验、病原菌形态学观察、分子生物学真菌r DNA-ITS序列分析,对病原菌进行鉴定,采用离体叶片接种方法对不同种属的植物叶片进行致病性测定,确定病原菌寄主范围。结果:从收集的栀子褐斑病病原菌感染的叶片中分离纯化得到的菌株,在培养基中初期气生菌丝绒毛状,白色,菌落的颜色初为白色,后变为深褐色,显微镜下观察菌丝有隔及分枝,子实体球型,灰色至褐色,分离的菌株回接后可使栀子叶片产生褐斑病病害,对扩增得到的r DNA-ITS序列进行Blast对比分析,确定该病害为葡萄座腔菌Botryosphaeria dothidea引起。病原菌寄主范围较广泛,供试的10种植物中对白花前胡的致病性最强,对千日红则没有致病性。结论:明确了栀子褐斑病病原菌及其寄主范围。
Objective： To clarify the pathogenic species of Gardenia jasminoides brown spot,and provide the basis for disease prevention and control. Method： The diseased leaf with G. jasminoides brown spot were collected from different regions of Jiangxi province. Pathogens were separated and purified by tissue culture method,and identified by using pathogenic test, pathological observation and r DNA-ITS sequence analysis according the Koch＇s postulates. Pathogenicity of the leaf from different species plants was identified by using isolated leaf inoculation method to determine the host range. Result： The pathogens separated from sample leaves showed white villiform aerial hyphae at the initial stage in medium,so that the color of colony was white at first,and then turned to dark brown. Observation under microscope showed that the hyphae had septum and branch,with gray to brown spheroidal fruiting body. The separated pathogen would make the healthy G. jasminoides leaves produce brown spot after tie-back. Blast analysis of the amplificated r DNA-ITS sequence confirmed that the disease was caused by Botryosphaeria dothidea. The pathogen had a wide host range,and the test results of 10 kinds of plants showed strongest pathogenicity in Peucedanum praeruptorum,but showed no pathogenicity in Gomphrena globosa. Conclusion： The pathogens and host range for G. jasminoides brown spot were clarified in this paper.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第23期35-39,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家公益性行业科研专项(201507002)
国家"重大新药创制"科技重大专项(2011BAI04B01)
中央本级重大增减支项目(20603020213
20603020110)
关键词
栀子
褐斑病
病原菌
Gardenia jasminoides
brown spot
pathogen
